Search Results - "Fernández‐Busnadiego, Rubén"

Three-dimensional architecture of extended synaptotagmin-mediated endoplasmic reticulum–plasma membrane contact sites by Fernández-Busnadiego, Rubén, Saheki, Yasunori, De Camilli, Pietro

“…The close apposition between the endoplasmic reticulum (ER) and the plasma membrane (PM) plays important roles in Ca ²⁺ homeostasis, signaling, and lipid…”
Get full text

Cryo‐electron tomography—the cell biology that came in from the cold by Wagner, Jonathan, Schaffer, Miroslava, Fernández‐Busnadiego, Rubén

“…Cryo‐electron tomography (cryo‐ET) provides high‐resolution 3D views into cells pristinely preserved by vitrification. Recent technical advances such as direct…”
Get full text

In Situ Structure of Neuronal C9orf72 Poly-GA Aggregates Reveals Proteasome Recruitment by Guo, Qiang, Lehmer, Carina, Martínez-Sánchez, Antonio, Rudack, Till, Beck, Florian, Hartmann, Hannelore, Pérez-Berlanga, Manuela, Frottin, Frédéric, Hipp, Mark S., Hartl, F. Ulrich, Edbauer, Dieter, Baumeister, Wolfgang, Fernández-Busnadiego, Rubén

“…Protein aggregation and dysfunction of the ubiquitin-proteasome system are hallmarks of many neurodegenerative diseases. Here, we address the elusive link…”
Get full text

In Situ Architecture and Cellular Interactions of PolyQ Inclusions by Bäuerlein, Felix J.B., Saha, Itika, Mishra, Archana, Kalemanov, Maria, Martínez-Sánchez, Antonio, Klein, Rüdiger, Dudanova, Irina, Hipp, Mark S., Hartl, F. Ulrich, Baumeister, Wolfgang, Fernández-Busnadiego, Rubén

“…Expression of many disease-related aggregation-prone proteins results in cytotoxicity and the formation of large intracellular inclusion bodies. To gain…”
Get full text

In situ architecture of neuronal α-Synuclein inclusions by Trinkaus, Victoria A., Riera-Tur, Irene, Martínez-Sánchez, Antonio, Bäuerlein, Felix J. B., Guo, Qiang, Arzberger, Thomas, Baumeister, Wolfgang, Dudanova, Irina, Hipp, Mark S., Hartl, F. Ulrich, Fernández-Busnadiego, Rubén

“…The molecular architecture of α-Synuclein (α-Syn) inclusions, pathognomonic of various neurodegenerative disorders, remains unclear. α-Syn inclusions were long…”
Get full text

Gel‐like inclusions of C‐terminal fragments of TDP‐43 sequester stalled proteasomes in neurons by Riemenschneider, Henrick, Guo, Qiang, Bader, Jakob, Frottin, Frédéric, Farny, Daniel, Kleinberger, Gernot, Haass, Christian, Mann, Matthias, Hartl, F. Ulrich, Baumeister, Wolfgang, Hipp, Mark S, Meissner, Felix, Fernández‐Busnadiego, Rubén, Edbauer, Dieter

“…Aggregation of the multifunctional RNA‐binding protein TDP‐43 defines large subgroups of amyotrophic lateral sclerosis and frontotemporal dementia and…”
Get full text

Tricalbin-Mediated Contact Sites Control ER Curvature to Maintain Plasma Membrane Integrity by Collado, Javier, Kalemanov, Maria, Campelo, Felix, Bourgoint, Clélia, Thomas, Ffion, Loewith, Robbie, Martínez-Sánchez, Antonio, Baumeister, Wolfgang, Stefan, Christopher J., Fernández-Busnadiego, Rubén

“…Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) play fundamental roles in all eukaryotic cells. ER-PM MCS are…”
Get full text

Reliable estimation of membrane curvature for cryo-electron tomography by Salfer, Maria, Collado, Javier F, Baumeister, Wolfgang, Fernandez-Busnadiego, Ruben, Martinez-Sanchez, Antonio

“…Curvature is a fundamental morphological descriptor of cellular membranes. Cryo-electron tomography (cryo-ET) is particularly well-suited to visualize and…”
Get full text

Molecular and structural architecture of polyQ aggregates in yeast by Gruber, Anselm, Hornburg, Daniel, Antonin, Matthias, Krahmer, Natalie, Collado, Javier, Schaffer, Miroslava, Zubaite, Greta, Lüchtenborg, Christian, Sachsenheimer, Timo, Brügger, Britta, Mann, Matthias, Baumeister, Wolfgang, Hartl, F. Ulrich, Hipp, Mark S., Fernández-Busnadiego, Rubén

“…Huntington’s disease is caused by the expansion of a polyglutamine (polyQ) tract in the N-terminal exon of huntingtin (HttEx1), but the cellular mechanisms…”
Get full text

Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography by Fernández-Busnadiego, Rubén, Zuber, Benoît, Maurer, Ulrike Elisabeth, Cyrklaff, Marek, Baumeister, Wolfgang, Lučić, Vladan

“…The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography…”
Get full text

Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits by Messa, Mirko, Fernández-Busnadiego, Rubén, Sun, Elizabeth Wen, Chen, Hong, Czapla, Heather, Wrasman, Kristie, Wu, Yumei, Ko, Genevieve, Ross, Theodora, Wendland, Beverly, De Camilli, Pietro

“…Epsin is an evolutionarily conserved endocytic clathrin adaptor whose most critical function(s) in clathrin coat dynamics remain(s) elusive. To elucidate such…”
Get full text

The synaptic vesicle cluster as a controller of pre- and postsynaptic structure and function by Reshetniak, Sofiia, Bogaciu, Cristian A, Bonn, Stefan, Brose, Nils, Cooper, Benjamin H, D'Este, Elisa, Fauth, Michael, Fernández-Busnadiego, Rubén, Fiosins, Maksims, Fischer, André, Georgiev, Svilen V, Jakobs, Stefan, Klumpp, Stefan, Köster, Sarah, Lange, Felix, Lipstein, Noa, Macarrón-Palacios, Victor, Milovanovic, Dragomir, Moser, Tobias, Müller, Marcus, Opazo, Felipe, Outeiro, Tiago F, Pape, Constantin, Priesemann, Viola, Rehling, Peter, Salditt, Tim, Schlüter, Oliver, Simeth, Nadja, Steinem, Claudia, Tchumatchenko, Tatjana, Tetzlaff, Christian, Tirard, Marilyn, Urlaub, Henning, Wichmann, Carolin, Wolf, Fred, Rizzoli, Silvio O

“…The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which provides neurotransmitter-loaded vesicles during synaptic activity, at…”
Get full text

Vesicle condensation induced by synapsin: condensate size, geometry, and vesicle shape deformations by Alfken, Jette, Neuhaus, Charlotte, Major, András, Taskina, Alyona, Hoffmann, Christian, Ganzella, Marcelo, Petrovic, Arsen, Zwicker, David, Fernández-Busnadiego, Rubén, Jahn, Reinhard, Milovanovic, Dragomir, Salditt, Tim

“…We study the formation of vesicle condensates induced by the protein synapsin, as a cell-free model system mimicking vesicle pool formation in the synapse. The…”
Get full text

Helical superstructures between amyloid and collagen in cardiac fibrils from a patient with AL amyloidosis by Schulte, Tim, Chaves-Sanjuan, Antonio, Speranzini, Valentina, Sicking, Kevin, Milazzo, Melissa, Mazzini, Giulia, Rognoni, Paola, Caminito, Serena, Milani, Paolo, Marabelli, Chiara, Corbelli, Alessandro, Diomede, Luisa, Fiordaliso, Fabio, Anastasia, Luigi, Pappone, Carlo, Merlini, Giampaolo, Bolognesi, Martino, Nuvolone, Mario, Fernández-Busnadiego, Rubén, Palladini, Giovanni, Ricagno, Stefano

“…Systemic light chain (LC) amyloidosis (AL) is a disease where organs are damaged by an overload of a misfolded patient-specific antibody-derived LC, secreted…”
Get full text

The evolution of the huntingtin-associated protein 40 (HAP40) in conjunction with huntingtin by Seefelder, Manuel, Alva, Vikram, Huang, Bin, Engler, Tatjana, Baumeister, Wolfgang, Guo, Qiang, Fernández-Busnadiego, Rubén, Lupas, Andrei N, Kochanek, Stefan

“…The huntingtin-associated protein 40 (HAP40) abundantly interacts with huntingtin (HTT), the protein that is altered in Huntington's disease (HD). Therefore,…”
Get full text

Quantitative Synaptic Biology: A Perspective on Techniques, Numbers and Expectations by Reshetniak, Sofiia, Fernández-Busnadiego, Rubén, Müller, Marcus, Rizzoli, Silvio O, Tetzlaff, Christian

“…Synapses play a central role for the processing of information in the brain and have been analyzed in countless biochemical, electrophysiological, imaging, and…”
Get full text

Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the nucleus by membranes associated with a novel nuclear pore structure by Goodwin, Edward C, Motamedi, Nasim, Lipovsky, Alex, Fernández-Busnadiego, Rubén, DiMaio, Daniel

“…DNAJB12 and DNAJB14 are transmembrane proteins in the endoplasmic reticulum (ER) that serve as co-chaperones for Hsc70/Hsp70 heat shock proteins. We…”
Get full text

In situ studies of membrane biology by cryo-electron tomography by Keller, Jenny, Fernández-Busnadiego, Rubén

“…Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of…”
Get full text

Stress- and ubiquitylation-dependent phase separation of the proteasome by Yasuda, Sayaka, Tsuchiya, Hikaru, Kaiho, Ai, Guo, Qiang, Ikeuchi, Ken, Endo, Akinori, Arai, Naoko, Ohtake, Fumiaki, Murata, Shigeo, Inada, Toshifumi, Baumeister, Wolfgang, Fernández-Busnadiego, Rubén, Tanaka, Keiji, Saeki, Yasushi

“…The proteasome is a major proteolytic machine that regulates cellular proteostasis through selective degradation of ubiquitylated proteins 1 , 2 . A number of…”
Get full text

Deciphering the molecular architecture of membrane contact sites by cryo-electron tomography by Collado, Javier, Fernández-Busnadiego, Rubén

“…At membrane contact sites (MCS) two cellular membranes form tight appositions that play critical roles in fundamental phenomena such as lipid metabolism or…”
Get full text