Search Results - "Fernández, Julio M."

Ephemeral states in protein folding under force captured with a magnetic tweezers design by Tapia-Rojo, Rafael, Eckels, Edward C., Fernández, Julio M.

“…Magnetic tape heads are ubiquitously used to read and record on magnetic tapes in technologies as diverse as old VHS tapes, modern hard-drive disks, or…”
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Talin folding as the tuning fork of cellular mechanotransduction by Tapia-Rojo, Rafael, Alonso-Caballero, Álvaro, Fernández, Julio M.

“…Cells continually sample their mechanical environment using exquisite force sensors such as talin, whose folding status triggers mechanotransduction pathways…”
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Work Done by Titin Protein Folding Assists Muscle Contraction by Rivas-Pardo, Jaime Andrés, Eckels, Edward C., Popa, Ionel, Kosuri, Pallav, Linke, Wolfgang A., Fernández, Julio M.

“…Current theories of muscle contraction propose that the power stroke of a myosin motor is the sole source of mechanical energy driving the sliding filaments of…”
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Mechanochemistry: One Bond at a Time by Liang, Jian, Fernández, Julio M

“…Single-molecule force-clamp spectroscopy offers a novel platform for mechanically denaturing proteins by applying a constant force to a polyprotein. A powerful…”
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The Work of Titin Protein Folding as a Major Driver in Muscle Contraction by Eckels, Edward C, Tapia-Rojo, Rafael, Rivas-Pardo, Jamie Andrés, Fernández, Julio M

“…Single-molecule atomic force microscopy and magnetic tweezers experiments have demonstrated that titin immunoglobulin (Ig) domains are capable of folding…”
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S-Glutathionylation of Cryptic Cysteines Enhances Titin Elasticity by Blocking Protein Folding by Alegre-Cebollada, Jorge, Kosuri, Pallav, Giganti, David, Eckels, Edward, Rivas-Pardo, Jaime Andrés, Hamdani, Nazha, Warren, Chad M., Solaro, R. John, Linke, Wolfgang A., Fernández, Julio M.

“…The giant elastic protein titin is a determinant factor in how much blood fills the left ventricle during diastole and thus in the etiology of heart disease…”
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A HaloTag Anchored Ruler for Week-Long Studies of Protein Dynamics by Popa, Ionel, Rivas-Pardo, Jaime Andrés, Eckels, Edward C, Echelman, Daniel J, Badilla, Carmen L, Valle-Orero, Jessica, Fernández, Julio M

“…Under physiological conditions, protein oxidation and misfolding occur with very low probability and on long times scales. Single-molecule techniques provide…”
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CnaA domains in bacterial pili are efficient dissipaters of large mechanical shocks by Echelman, Daniel J., Alegre-Cebollada, Jorge, Badilla, Carmen L., Chang, Chungyu, Ton-That, Hung, Fernández, Julio M.

“…Pathogenic bacteria adhere despite severe mechanical perturbations induced by the host, such as coughing. In Gram-positive bacteria, extracellular protein…”
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Segmentation and the Entropic Elasticity of Modular Proteins by Berkovich, Ronen, Fernandez, Vicente I, Stirnemann, Guillaume, Valle-Orero, Jessica, Fernández, Julio M

“…Single-molecule force spectroscopy utilizes polyproteins, which are composed of tandem modular domains, to study their mechanical and structural properties…”
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Direct observation of disulfide isomerization in a single protein by Alegre-Cebollada, Jorge, Kosuri, Pallav, Rivas-Pardo, Jaime Andrés, Fernández, Julio M.

“…Photochemical uncaging techniques use light to release active molecules from otherwise inert compounds. Here we expand this class of techniques by…”
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The Mechanical Power of Titin Folding by Eckels, Edward C., Haldar, Shubhasis, Tapia-Rojo, Rafael, Rivas-Pardo, Jaime Andrés, Fernández, Julio M.

“…The delivery of mechanical power, a crucial component of animal motion, is constrained by the universal compromise between the force and the velocity of its…”
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Kinetic Measurements on Single-Molecule Disulfide Bond Cleavage by Liang, Jian, Fernández, Julio M

“…We use single-molecule force clamp spectroscopy (SMFCS) to explore the reactivity of tris(2-carboxyethyl)phosphine (TCEP), 1, 4-dl-dithiothreitol (DTT) and…”
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Direct Quantification of the Attempt Frequency Determining the Mechanical Unfolding of Ubiquitin Protein by Popa, Ionel, Fernández, Julio M., Garcia-Manyes, Sergi

“…Understanding protein dynamics requires a comprehensive knowledge of the underlying potential energy surface that governs the motion of each individual protein…”
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Isopeptide Bonds Block the Mechanical Extension of Pili in Pathogenic Streptococcus pyogenes by Alegre-Cebollada, Jorge, Badilla, Carmen L., Fernández, Julio M.

“…In the early stages of an infection, pathogenic bacteria use long fibrous structures known as pili as adhesive anchors for attachment to the host cells. These…”
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Mechanical forces regulate the reactivity of a thioester bond in a bacterial adhesin by Echelman, Daniel J., Lee, Alex Q., Fernández, Julio M.

“…Bacteria must withstand large mechanical shear forces when adhering to and colonizing hosts. Recent structural studies on a class of Gram-positive bacterial…”
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Mechanical Deformation Accelerates Protein Ageing by Valle‐Orero, Jessica, Rivas‐Pardo, Jaime Andrés, Tapia‐Rojo, Rafael, Popa, Ionel, Echelman, Daniel J., Haldar, Shubhasis, Fernández, Julio M.

“…A hallmark of tissue ageing is the irreversible oxidative modification of its proteins. We show that single proteins, kept unfolded and extended by a…”
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Force-Clamp Spectroscopy of Single-Protein Monomers Reveals the Individual Unfolding and Folding Pathways of I27 and Ubiquitin by Garcia-Manyes, Sergi, Bruji&cacute, Jasna, Badilla, Carmen L., Fernández, Julio M.

“…Single-protein force experiments have relied on a molecular fingerprint based on tethering multiple single-protein domains in a polyprotein chain. However,…”
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Force-activated reactivity switch in a bimolecular chemical reaction by Garcia-Manyes, Sergi, Fernández, Julio M, Liang, Jian, Szoszkiewicz, Robert, Kuo, Tzu-Ling

“…The effect of mechanical force on the free-energy surface that governs a chemical reaction is largely unknown. The combination of protein engineering with…”
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Proteins Breaking Bad: A Free Energy Perspective by Valle-Orero, Jessica, Tapia-Rojo, Rafael, Eckels, Edward C, Rivas-Pardo, Jaime Andrés, Popa, Ionel, Fernández, Julio M

“…Protein aging may manifest as a mechanical disease that compromises tissue elasticity. As proved recently, while proteins respond to changes in force with an…”
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Monitoring Oxidative Folding of a Single Protein Catalyzed by the Disulfide Oxidoreductase DsbA by Kahn, Thomas B., Fernández, Julio M., Perez-Jimenez, Raul

“…Oxidative folding, the process by which proteins fold and acquire disulfide bonds concurrently, is of critical importance for a wide range of biological…”
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