Virulence determinants, biofilm production and antimicrobial susceptibility in Staphylococcus aureus causing device-associated infections in a Tunisian hospital

•The high virulence potential of Staphylococcus aureus strains causing device-related infections (DRI).•The multi-drug-resistance pattern of meticillin-resistant S. aureus isolated from biomaterials.•The epidemic spread of S. aureus clones causing DRI in the hospital. Staphylococcus aureus is a clin...

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Bibliographic Details
Published in:	International journal of antimicrobial agents Vol. 52; no. 6; pp. 922 - 929
Main Authors:	Mesrati, I., Saidani, M., Jemili, M., Ferjeni, S., Slim, A., Boubaker, I. Boutiba-Ben
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-12-2018
Subjects:	Antibiotic resistance Biofilm formation Device-related infections Staphylococcus aureus Virulence genes Device-related infections Virulence genes Biofilm formation Staphylococcus aureus Antibiotic resistance
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Summary:	•The high virulence potential of Staphylococcus aureus strains causing device-related infections (DRI).•The multi-drug-resistance pattern of meticillin-resistant S. aureus isolated from biomaterials.•The epidemic spread of S. aureus clones causing DRI in the hospital. Staphylococcus aureus is a clinically relevant pathogen that causes device-related infections (DRI) driven by several virulence factors. This study characterized S. aureus isolates involved in DRI in Tunisian patients. Forty consecutive S. aureus strains causing DRI and 47 randomly selected S. aureus strains causing non-device-related infections (NDRI) were collected. All strains were screened phenotypically for antibiotic susceptibility and biofilm forming ability. They were investigated for accessory gene regulator (agr) types, biofilm encoding genes (icaADBC), adhesins, leukotoxins, toxic shock toxin, enterotoxins and exotoxins encoding genes by polymerase chain reaction. Meticillin-resistant S. aureus (MRSA) strains were further characterized by staphylococcal cassette chromosome mec (SCCmec) typing. MRSA rates among DRI and NDRI isolates were 23% and 49% (P=0.02), respectively. The DRI isolates formed biofilm more frequently (n=32) than the NDRI isolates (n=28) (P=0.04), with predominance of the moderate biofilm producer category (P=0.027). All biofilm-positive isolates except four harboured icaADBC genes. A significant difference was observed between DRI and NDRI isolates for fnbA (53–77%), spa (45–26%), sdrD (80–55%) and sen (33–11%) genes. DRI strains were agrI (48%) and agrII (30%) types, whereas NDRI strains were agrI (36%) and agrIII (43%) types. SCCmec type IV was carried by 50% of MRSA isolates. This study highlights the virulence potential displayed by S. aureus isolated from DRI in comparison with NDRI.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0924-8579 1872-7913
DOI:	10.1016/j.ijantimicag.2018.05.004