Association of breast cancer with human papillomavirus (HPV) infection in Northeast Brazil: molecular evidence

The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant control...

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Bibliographic Details
Published in:	Clinics (São Paulo, Brazil) Vol. 73; p. e465
Main Authors:	Cavalcante, José Roosevelt, Pinheiro, Luiz Gonzaga Porto, de Almeida, Paulo Roberto Carvalho, Ferreira, Márcia Valéria Pitombeira, Cruz, Gizele Almada, Campelo, Thales Alves, Silva, Caroliny Soares, Lima, Luana Nepomuceno Gondim Costa, de Oliveira, Bruno Masato Kitagawa, Lima, Lara Mulato, Feitosa, Laura Magda Costa, Pinheiro, Agostinho Câmara, Frota, Cristiane Cunha
Format:	Journal Article
Language:	English
Published:	Brazil Elsevier España, S.L.U 01-01-2018 Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo Faculdade de Medicina / USP Elsevier España
Subjects:	Adult Aged Aged, 80 and over Breast Cancer Breast Neoplasms Breast Neoplasms - virology Case-Control Studies Cross-Sectional Studies DNA, Viral - genetics Female Genotype HPV Human Papillomavirus Humans MEDICINE, GENERAL & INTERNAL Middle Aged Molecular Typing Original Papillomavirus Infections - genetics Polymerase Chain Reaction Human Papillomavirus Breast Neoplasms Breast Cancer Molecular Typing HPV
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Summary:	The aim of this study is to investigate the presence of human papillomavirus DNA and genotypes in breast cancer and normal breast tissue samples obtained from women from the northeast region of Brazil. One hundred three breast cancer samples and 95 normal breast samples, as the non-malignant controls, were studied. DNA extraction was verified by human beta-globin gene amplification, and polymerase chain reaction was conducted based on HPV L1-specific consensus primers MY09/MY11 and GP5+/GP6+, followed by nested multiplex polymerase chain reaction with type-specific primers for the E6/E7 consensus region. Human papillomavirus DNA was detected in 51 (49.5%) breast carcinoma samples and 15 (15.8%) normal breast samples (p<0.0001). Human papillomavirus genotypes 6 and 11 were identified in 15.2% of all samples. The high frequency of human papillomavirus infection in breast cancer samples indicates a potential role of this virus in breast carcinogenesis in the studied participants.
ISSN:	1807-5932 1980-5322 1980-5322
DOI:	10.6061/clinics/2018/e465