Variable responses of different human cancer cells to the lichen compounds parietin, atranorin, usnic acid and gyrophoric acid

One of the ways for searching for potentially new anti-cancer drugs is the testing of various naturally synthesized compounds. Lichens are a source of unique chemical agents of which some have already been proved to be effective against various cancer in vitro models. Our study reports on the sensit...

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Bibliographic Details
Published in:	Toxicology in vitro Vol. 25; no. 1; pp. 37 - 44
Main Authors:	BaAekorova, M, BaAekor, M, Mikes, J, Jendzelovsky, R, FedoroAeko, P
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-02-2011
Subjects:	Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Benzoates - pharmacology Benzofurans - pharmacology Cancer cell Cell Adhesion - drug effects Cell cycle Cell Line, Tumor Cell Nucleus Shape - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Cytotoxicity Drug Discovery Drug Screening Assays, Antitumor Emodin - analogs & derivatives Emodin - pharmacology Humans Hydroxybenzoates - pharmacology Lichen metabolites Lichens - chemistry Neoplasms - drug therapy Neoplasms - pathology Osmolar Concentration S Phase - drug effects Cancer cell Lichen metabolites Cytotoxicity Cell cycle
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Summary:	One of the ways for searching for potentially new anti-cancer drugs is the testing of various naturally synthesized compounds. Lichens are a source of unique chemical agents of which some have already been proved to be effective against various cancer in vitro models. Our study reports on the sensitivity of up to nine human cancer cell lines (A2780, HeLa, MCF-7, SK-BR-3, HT-29, HCT-116 p53 +/+, HCT-116 p53 −/−, HL-60 and Jurkat) to the anti-proliferative/cytotoxic effects of four typical secondary metabolites of lichens (parietin, atranorin, usnic acid and gyrophoric acid). Variations in the dynamics of tumour cell line populations were evaluated by the MTT, clonogenic and viability assays, cell proliferation and detachment, cell cycle transition and apoptotic nuclear morphology, thereby confirming their concentration- and time-dependent cytotoxicity. However, in comparison with parietin and gyrophoric acid, the suppression of viability and cell proliferation by usnic acid or atranorin was found to be more efficient at equitoxic doses and correlated more strongly with an increased number of floating cells or a higher apoptotic index. Moreover, the analysis of cell cycle distribution also revealed an accumulation of cells in S-phase. This study has confirmed a differential sensitivity of cancer cell lines to lichen secondary metabolites.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0887-2333 1879-3177
DOI:	10.1016/j.tiv.2010.09.004