Development of a rapid LC-MS/MS method for simultaneous quantification of ten commonly used antibiotic drugs in human serum

The use of TDM in clinical practice to monitor the plasma levels of antibiotics administered to critically ill patients is a well-established approach that allows for optimization of the patient's response to drug therapy, considering the characteristics of the drug, the clinical and physiologi...

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Bibliographic Details
Published in:	Journal of pharmaceutical and biomedical analysis Vol. 244; p. 116119
Main Authors:	Ramirez, Stefania, Scapaticci, Margherita, Barbella, Francesca, Panico, M. Marcella, Fecca, Ileana A., Cocchini, Barbara, Brizzi, Nadia, Cavina, Sonia, Accorsi, Irene, Rizzato, Silvia, Ramazzotti, Eric, Mancini, Rita
Format:	Journal Article
Language:	English
Published:	England Elsevier B.V 15-07-2024
Subjects:	Anti-Bacterial Agents - blood Antibiotics Drug Monitoring - methods Humans Internal validation LC-MS/MS Liquid Chromatography-Mass Spectrometry - methods Real-time TDM Reproducibility of Results Tandem Mass Spectrometry - methods LC-MS/MS Antibiotics Internal validation Real-time TDM
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Summary:	The use of TDM in clinical practice to monitor the plasma levels of antibiotics administered to critically ill patients is a well-established approach that allows for optimization of the patient's response to drug therapy, considering the characteristics of the drug, the clinical and physiological status of the patient and any peculiar of the pathogen that caused the clinical picture. In our laboratory, we have developed a single LC-MS/MS analysis for dosing the serum concentration of an antibacterial panel composed of eight antibacterial and two selective inhibitors. The method presented used a certified material furnished by a commercial company and was internally validated using the EMA guidelines. The results have shown high sensitivity, precision, and accuracy, a lower matrix effect combined with simple sample preparation and a time-saving procedure. We have evaluated the recovery rate and matrix effect by testing serum samples without pathological index and serum pools obtained from haemolysed, icteric, or lipemic samples. The assay has shown a recovery range between 94% and 101%. •The tdm allows rapid adaptation of therapy in hospitalized subjects.•Personalized therapy is essential for the management of the critical patient.•Mass spectrometry is the pioneering technique in the correct determination of blood concentrations of drugs.•Validation of analytical methods is mandatory for the use of a method in diagnostics.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0731-7085 1873-264X 1873-264X
DOI:	10.1016/j.jpba.2024.116119