Decidual Differentiation of Stromal Cells Promotes Proprotein Convertase 5/6 Expression and Lefty Processing

Decidual Differentiation of Stromal Cells Promotes Proprotein Convertase 5/6 Expression and Lefty Processing

Lefty/Ebaf polypeptides, novel members of the TGF-β superfamily, are involved in endometrial differentiation and embryo implantation. Recently, we showed that, during undisturbed estrous cycle, lefty is present in mouse uterine horn primarily in a precursor form. Here, we show that decidual differen...

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Bibliographic Details
Published in:Endocrinology (Philadelphia) Vol. 146; no. 12; pp. 5313 - 5320
Main Authors: Tang, Meiyi, Mikhailik, Anatoly, Pauli, Ilse, Giudice, Linda C, Fazelabas, Asgerally T, Tulac, Suzana, Carson, Daniel D, Kaufman, David G, Barbier, Claire, Creemers, John W. M, Tabibzadeh, Siamak
Format: Journal Article
Language:English
Published: Bethesda, MD Endocrine Society 01-12-2005
Oxford University Press
Subjects:
Animals
Biological and medical sciences
Cell differentiation
Cell Differentiation - physiology
Cells, Cultured
Decidua
Decidua - physiology
Differentiation
Embryos
Endometrium
Endometrium - cytology
Estrus cycle
Female
Fundamental and applied biological sciences. Psychology
Implantation
Left-Right Determination Factors
Mice
Polypeptides
Precursors
Pregnancy
Proprotein Convertase 5 - metabolism
Proprotein convertases
Protein Processing, Post-Translational
Stromal cells
Stromal Cells - cytology
Transforming Growth Factor beta - genetics
Transforming growth factor-b
Uterus
Vertebrates: endocrinology
Cell differentiation
Stromal cell
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Summary:Lefty/Ebaf polypeptides, novel members of the TGF-β superfamily, are involved in endometrial differentiation and embryo implantation. Recently, we showed that, during undisturbed estrous cycle, lefty is present in mouse uterine horn primarily in a precursor form. Here, we show that decidual differentiation of endometrial stroma leads to increased lefty (∼3.1- to 3.6-fold in vivo and 5- to 8-fold in vitro) and processing of its precursor primarily to its long form. This event occurs on d 5 of pregnancy, and is paralleled by proprotein convertase (PC)5/6 up-regulation (∼6-fold increase for PC5A and 3-fold increase for PC5B) in decidualized uterine horn, independent of embryo implantation. Among the known convertases, only PC5/6A processes lefty to its long form. Taken together, the findings show that decidualized differentiation of stroma, which is a prerequisite for embryo implantation, leads to processing of lefty by PC5/6A.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0013-7227
1945-7170
DOI:10.1210/en.2005-0684