Some factors affecting production of pectic enzymes by Aspergillus niger
The effects of varying cultural conditions were assessed for the production of pectic enzymes in a strain of Aspergillus niger, isolated from decaying orange fruit. Polygalacturonase and pectinmethylesterase were found to be inducible by polygalacturonic acid and pectin in the medium, respectively....
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Published in: | International biodeterioration & biodegradation Vol. 52; no. 4; pp. 223 - 227 |
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Main Authors: | , |
Format: | Journal Article |
Language: | English |
Published: |
Barking
Elsevier Ltd
01-01-2003
Elsevier |
Subjects: | |
Online Access: | Get full text |
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Summary: | The effects of varying cultural conditions were assessed for the production of pectic enzymes in a strain of
Aspergillus niger, isolated from decaying orange fruit. Polygalacturonase and pectinmethylesterase were found to be inducible by polygalacturonic acid and pectin in the medium, respectively. Ammonium sulphate was the best nitrogen source for the production of both enzymes. There were variations in enzyme levels produced in culture filtrates with age of the culture, the highest levels being in 4-day-old cultures. The temperature and pH also had marked effects on the production of pectic enzymes with the best conditions being 40°C and pH 5, respectively. Surface culture technique gave appreciable enzyme yield, while agitation had an inhibitory effect on enzyme production. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0964-8305 1879-0208 |
DOI: | 10.1016/S0964-8305(03)00094-5 |