Genome-Wide Analysis of Differentially Expressed Genes During the Early Stages of Tomato Infection by a Potyvirus

Plant responses against pathogens cause up- and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from...

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Bibliographic Details
Published in:	Molecular plant-microbe interactions Vol. 22; no. 3; pp. 352 - 361
Main Authors:	Alfenas-Zerbini, Poliane, Maia, Ivan G, Favaro, Regiane D, Cascardo, Julio C.M, Brommonschenkel, Sergio H, Zerbini, Francisco Murilo
Format:	Journal Article
Language:	English
Published:	St. Paul, MN APS Press 01-03-2009 The American Phytopathological Society
Subjects:	biochemical pathways Biological and medical sciences cell cycle disease course disease resistance Fundamental and applied biological sciences. Psychology gene expression Gene Expression Profiling gene expression regulation Gene Expression Regulation, Plant - physiology genome Genome, Plant genomics infection Lycopersicon esculentum Lycopersicon esculentum - metabolism Lycopersicon esculentum - virology pathogenesis-related proteins Pepper yellow mosaic virus Phytopathology. Animal pests. Plant and forest protection Plant Diseases plant diseases and disorders Plant Leaves - virology Plant Proteins - genetics Plant Proteins - metabolism plant viruses Plant viruses and viroids Potyvirus Potyvirus - physiology resistance mechanisms signal transduction Solanum lycopersicum var. lycopersicum tomatoes transcription (genetics) ubiquitin Plant pathogen Microbiology Early stage Virus Vegetable crop Gene Dicotyledones Angiospermae Lycopersicon esculentum Spermatophyta Solanaceae Potyvirus Genome Potyviridae
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Summary:	Plant responses against pathogens cause up- and downward shifts in gene expression. To identify differentially expressed genes in a plant-virus interaction, susceptible tomato plants were inoculated with the potyvirus Pepper yellow mosaic virus (PepYMV) and a subtractive library was constructed from inoculated leaves at 72 h after inoculation. Several genes were identified as upregulated, including genes involved in plant defense responses (e.g., pathogenesis-related protein 5), regulation of the cell cycle (e.g., cytokinin-repressed proteins), signal transduction (e.g., CAX-interacting protein 4, SNF1 kinase), transcriptional regulators (e.g., WRKY and SCARECROW transcription factors), stress response proteins (e.g., Hsp90, DNA-J, 20S proteasome alpha subunit B, translationally controlled tumor protein), ubiquitins (e.g., polyubiquitin, ubiquitin activating enzyme 2), among others. Downregulated genes were also identified, which likewise display identity with genes involved in several metabolic pathways. Differential expression of selected genes was validated by macroarray analysis and quantitative real-time polymerase chain reaction. The possible roles played by some of these genes in the viral infection cycle are discussed.
Bibliography:	http://dx.doi.org/10.1094/MPMI-22-3-0352 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0894-0282 1943-7706
DOI:	10.1094/MPMI-22-3-0352