Identification of a second human acetyl-CoA carboxylase gene

Identification of a second human acetyl-CoA carboxylase gene

Acetyl-CoA carboxylase (ACC), an important enzyme in fatty acid biosynthesis and a regulator of fatty acid oxidation, is present in at least two isoenzymic forms in rat and human tissues. Previous work has established the existence of a 265,000 Da enzyme in both the rat and human (RACC265; HACC265)...

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Bibliographic Details
Published in:Biochemical journal Vol. 316 ( Pt 3); no. 3; pp. 915 - 922
Main Authors: Widmer, J, Fassihi, K S, Schlichter, S C, Wheeler, K S, Crute, B E, King, N, Nutile-McMenemy, N, Noll, W W, Daniel, S, Ha, J, Kim, K H, Witters, L A
Format: Journal Article
Language:English
Published: England 15-06-1996
Subjects:
Acetyl-CoA Carboxylase - biosynthesis
Acetyl-CoA Carboxylase - genetics
Amino Acid Sequence
Animals
Base Sequence
Cell Differentiation
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 17
Clofibric Acid - analogs & derivatives
Clofibric Acid - pharmacology
Cricetinae
Enzyme Induction - drug effects
Fibric Acids
Glucose - pharmacology
Humans
Isoenzymes - biosynthesis
Isoenzymes - genetics
Liver Neoplasms, Experimental
Molecular Sequence Data
Rats
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Transcription, Genetic
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Summary:Acetyl-CoA carboxylase (ACC), an important enzyme in fatty acid biosynthesis and a regulator of fatty acid oxidation, is present in at least two isoenzymic forms in rat and human tissues. Previous work has established the existence of a 265,000 Da enzyme in both the rat and human (RACC265; HACC265) and a higher-molecular-mass species (275,000-280,000 Da) in the same species (RACC280; HACC275). An HACC265 gene has previously been localized to chromosome 17. In the present study, we report cloning of a partial-length human cDNA sequence which appears to correspond to HACC275 and its rat homologue, RACC280, as judged by mRNA tissue distribution and cell-specific regulation of mRNA/protein expression. The gene encoding this isoenzymic form of ACC has been localized to the long arm of human chromosome 12. Thus, ACC is represented in a multigene family in both rodents and humans. The newly discovered human gene and its rat homologue appear to be under different regulatory control to the HACC265 gene, as judged by tissue-specific expression in vivo and by independent modulation in cultured cells in vitro.
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content type line 23
ISSN:0264-6021
1470-8728
DOI:10.1042/bj3160915