Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas

Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this stu...

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Bibliographic Details
Published in:	Scientific reports Vol. 14; no. 1; p. 10136
Main Authors:	Salg, Gabriel Alexander, Steinle, Verena, Labode, Jonas, Wagner, Willi, Studier-Fischer, Alexander, Reiser, Johanna, Farjallah, Elyes, Guettlein, Michelle, Albers, Jonas, Hilgenfeld, Tim, Giese, Nathalia A., Stiller, Wolfram, Nickel, Felix, Loos, Martin, Michalski, Christoph W., Kauczor, Hans-Ulrich, Hackert, Thilo, Dullin, Christian, Mayer, Philipp, Kenngott, Hannes Goetz
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 02-05-2024 Nature Publishing Group Nature Portfolio
Subjects:	631/1647/245 631/1647/245/1847 692/4020/2741/416 Animals Capillaries Computed tomography Histology Humanities and Social Sciences Imaging Imaging, Three-Dimensional - methods Immunohistochemistry Islets of Langerhans Islets of Langerhans - blood supply Islets of Langerhans - diagnostic imaging multidisciplinary Multimodal Imaging - methods Pancreas Pancreas - blood supply Pancreas - diagnostic imaging Science Science (multidisciplinary) Swine Synchrotron Tomography Tomography, X-Ray Computed - methods Vascularization Virtual histology X-Ray Microtomography - methods Computed tomography Imaging Virtual histology Islets of Langerhans Pancreas Synchrotron Vascularization
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Summary:	Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-024-60254-9