Antimicrobial, Antibiofilm Activities and Synergic Effect of Triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene Isolated from Combretum leprosum Leaves Against Staphylococcus Strains

Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of...

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Published in:	Current microbiology Vol. 80; no. 5; p. 176
Main Authors:	Teixeira, Edson Holanda, Andrade, Alexandre Lopes, Pereira, Rafael, Farias, Livia Pontes, Monteiro, Gabrieli Sobral, Marinho, Marcia Machado, Marinho, Emmanuel Silva, Santos, Hélcio Silva, de Vasconcelos, Mayron Alves
Format:	Journal Article
Language:	English
Published:	New York Springer US 01-05-2023 Springer Nature B.V
Subjects:	Ampicillin Ampicillin - pharmacology Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics Antimicrobial agents Antimicrobial resistance Bactericidal activity Biofilms Biomedical and Life Sciences Biotechnology Cell morphology Combretum - chemistry Cytology Drug resistance Enumeration Evaluation Gentian violet Life Sciences Methicillin Methicillin-Resistant Staphylococcus aureus Microbial Sensitivity Tests Microbiology Microorganisms Minimum inhibitory concentration Molecular docking Molecular Docking Simulation Penicillin Penicillin-binding protein Plant Extracts - pharmacology Proteins Public health Scanning electron microscopy Staphylococcus Staphylococcus aureus Staphylococcus epidermidis Strains (organisms) Synergistic effect Triterpenes - pharmacology
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Summary:	Antimicrobial resistance is a natural phenomenon and is becoming a huge global public health problem, since some microorganisms not respond to the treatment of several classes of antibiotics. The objective of the present study was to evaluate the antibacterial, antibiofilm, and synergistic effect of triterpene 3β,6β,16β-trihydroxyilup-20(29)-ene (CLF1) against Staphylococcus aureus and Staphylococcus epidermidis strains. Bacterial susceptibility to CLF1 was evaluated by minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) assay. In addition, the effect combined with antibiotics (ampicillin and tetracycline) was verified by the checkerboard method. The biofilms susceptibility was assessed by enumeration of colony-forming units (CFUs) and quantification of total biomass by crystal violet staining. The compound showed bacteriostatic and bactericidal activity against all Staphylococcal strains tested. The synergistic effect with ampicillin was observed only for S. epidermidis strains. Moreover, CLF1 significantly inhibited the biofilm formation and disrupted preformed biofilm of the all strains. Scanning electron microscopy (SEM) images showed changes in the cell morphology and structure of S. aureus ATCC 700698 biofilms (a methicillin-resistant S. aureus strain). Molecular docking simulations showed that CLF1 has a more favorable interaction energy than the antibiotic ampicillin on penicillin-binding protein (PBP) 2a of MRSA, coupled in different regions of the protein. Based on the results obtained, CLF1 proved to be a promising antimicrobial compound against Staphylococcus biofilms.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0343-8651 1432-0991
DOI:	10.1007/s00284-023-03284-2