Modulation of Inflammatory Processes by Leaves Extract from Clusia nemorosa Both In Vitro and In Vivo Animal Models

The present study was carried out to investigate the anti-inflammatory effect of the hexane extract of the leaves from Clusia nemorosa G. Mey, called HECn, using carrageenan-induced mice pleurisy and cotton pellet-induced mice granuloma. Additionally, the ability of HECn to affect both neutrophil mi...

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Bibliographic Details
Published in:	Inflammation Vol. 35; no. 2; pp. 764 - 771
Main Authors:	Farias, José Alex C., Ferro, Jamylle Nunes S., Silva, Juliane P., Agra, Isabela Karine R., Oliveira, Fernando M., Candea, André Luiz P., Conte, Fernando P., Ferraris, Fausto K., Henriques, Maria das Graças M. O., Conserva, Lucia M., Barreto, Emiliano
Format:	Journal Article
Language:	English
Published:	Boston Springer US 01-04-2012 Springer Nature B.V
Subjects:	Animals Anti-Inflammatory Agents - pharmacology Apoptosis - drug effects Biomedical and Life Sciences Biomedicine Carrageenan Cells, Cultured Chemotaxis, Leukocyte - drug effects Clusia Cotton Fiber Granuloma - chemically induced Granuloma - drug therapy Granuloma - immunology Humans Immunology Internal Medicine Mice Neutrophils - immunology Neutrophils - physiology Pathology Pharmacology/Toxicology Phytotherapy Plant Extracts - pharmacology Plant Leaves Pleurisy - chemically induced Pleurisy - drug therapy Pleurisy - immunology Rheumatology Tumor Necrosis Factor-alpha - biosynthesis anti-inflammatory effect neutrophil pleurisy chemotaxis
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Summary:	The present study was carried out to investigate the anti-inflammatory effect of the hexane extract of the leaves from Clusia nemorosa G. Mey, called HECn, using carrageenan-induced mice pleurisy and cotton pellet-induced mice granuloma. Additionally, the ability of HECn to affect both neutrophil migration as viability was investigated by use of the Boyden chamber assay and flow cytometry, respectively. The HECn significantly inhibited exudation, total leukocytes and neutrophils influx, as well as TNFα levels in carrageenan-induced pleurisy. However, the extract not suppressed the granulomatous tissue formation in the cotton pellet-induced granuloma test. Experiments performed in vitro revealed that HECn on human neutrophils inhibited a dose-dependent manner the CXCL1-induced neutrophil chemotaxis. Furthermore, HECn also inhibited the chemoattraction of human neutrophils induced by formyl-methionyl-leucyl-phenylalanine (fMLP), leukotriene B4 (LTB4) and platelet activating factor (PAF) in a Boyden chamber. However, this same treatment not was able to induce apoptosis. The results obtained in this study showed that the extract from leaves of C. nemorosa possess a potent inhibitory activity in acute model of inflammation, being the effects mediated, in part, by inhibition of neutrophil responsiveness. These results indicate that C. nemorosa could be a good source for anti-inflammatory compounds.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0360-3997 1573-2576
DOI:	10.1007/s10753-011-9372-y