Trypanosoma cruzi: The role of PGE2 in immune response during the acute phase of experimental infection

Chagas disease is characterized by cardiac lesions and a high level of PGE2. Our objective was to investigate the role of PGE2 in cardiac lesions. BALB/c mice were infected with Trypanosoma cruzi (1 × 10 3 trypomastigote forms) and were treated daily with PBS, meloxicam (0.5 mg/kg) or etoricoxib (0....

Full description

Saved in:

Bibliographic Details
Published in:	Experimental parasitology Vol. 118; no. 4; pp. 514 - 521
Main Authors:	Abdalla, G.K., Faria, G.E.L., Silva, K.T., Castro, E.C.C., Reis, M.A., Michelin, M.A.
Format:	Journal Article
Language:	English
Published:	San Diego, CA Elsevier Inc 01-04-2008 Elsevier
Subjects:	Acute Disease Animals Biological and medical sciences bovine serum albumin BSA Cardiac lesions Cells, Cultured Chagas Disease - drug therapy Chagas Disease - immunology Chagas Disease - pathology COX COX-2 cyclooxygenase Cyclooxygenase 1 - analysis Cyclooxygenase 2 - analysis Cyclooxygenase Inhibitors - therapeutic use Cytokines degree Celsius Dinoprostone - biosynthesis Dinoprostone - physiology Fibrosis Fundamental and applied biological sciences. Psychology hematoxylin–eosin hour IFN-γ immunoglobulin interferon γ interleukin Life cycle. Host-agent relationship. Pathogenesis major histocompatibility complex Male mg/kg MHC Mice Mice, Inbred BALB C micro Currier micrograms per milliliter milligrams per kilogram Myocardium - enzymology Myocardium - pathology nanograms per milliliter ng/ml nitric oxide Parasitemia - drug therapy Parasitemia - parasitology PBS PGE 2 phosphate buffered saline Prostaglandin prostaglandin E 2 Prostaglandins - analysis Protozoa Pyridines - therapeutic use Spleen - chemistry Spleen - cytology Sulfones - therapeutic use T helper T. cruzi Thiazines - therapeutic use Thiazoles - therapeutic use TNF-α Trypanosoma cruzi Trypanosoma cruzi - immunology tumor necrosis factor α μCi μg/ml PBS NO IL C Prostaglandin Cytokines COX-2 PGE 2 h ng/ml MHC T. cruzi μCi μg/ml COX BSA Cardiac lesions IFN-γ TNF-α Th HE Ig mg/kg Kinetoplastida Infection Protozoa Immune response Acute Parasite Organism defense Experimental study Trypanosoma cruzi
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Chagas disease is characterized by cardiac lesions and a high level of PGE2. Our objective was to investigate the role of PGE2 in cardiac lesions. BALB/c mice were infected with Trypanosoma cruzi (1 × 10 3 trypomastigote forms) and were treated daily with PBS, meloxicam (0.5 mg/kg) or etoricoxib (0.6 mg/kg). The animals were sacrificed on the 21st day of infection and we collected the cardiac tissue and spleen cells for tissue culture. We observed that treatment with COX-2 inhibitors was able to decrease synthesis of PGE2 by spleen cells. This reduction was accompanied by reduction of the inflammatory infiltrate, parasite nets, cardiac fibrosis and fewer COX-2 positive cells in cardiac tissue obtained from these animals. In conclusion, treatment with COX-2 inhibitors, and consequent inhibition of PGE2 synthesis, was able to reduce the cardiac damage observed during the acute phase of experimental Chagas disease, thus demonstrating the involvement of this mediator in the cardiac lesion.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0014-4894 1090-2449
DOI:	10.1016/j.exppara.2007.11.003