Disposable electrochemical genosensor for the simultaneous analysis of different bacterial food contaminants

Disposable electrochemical genosensor for the simultaneous analysis of different bacterial food contaminants

This paper deals with the use of an electrochemical genosensor array for the rapid and simultaneous detection of different food-contaminating pathogenic bacteria. The method includes PCR amplification followed by analysis of the amplicons by hybridisation with toxin-specific oligonucleotide probes....

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Bibliographic Details
Published in:Biosensors & bioelectronics Vol. 22; no. 7; pp. 1544 - 1549
Main Authors: Farabullini, F., Lucarelli, F., Palchetti, I., Marrazza, G., Mascini, M.
Format: Journal Article
Language:English
Published: Lausanne Elsevier B.V 15-02-2007
Elsevier Science
Subjects:
Bacteria
Bacteria - genetics
Bacteria - isolation & purification
Base Sequence
Biological and medical sciences
Biosensing Techniques - instrumentation
Biotechnology
DNA hybridisation
Electrochemical genosensor
Electrochemistry - instrumentation
Escherichia coli - genetics
Escherichia coli O157 - genetics
Food Microbiology
Fundamental and applied biological sciences. Psychology
Listeria monocytogenes - genetics
Molecular Sequence Data
PCR
Salmonella - genetics
Salmonella - isolation & purification
Screen-printed array
Staphylococcus aureus - genetics
Electrochemical genosensor
Bacteria
DNA hybridisation
Screen-printed array
PCR
Disposable
Molecular hybridization
Foodstuff
Polymerase chain reaction
Array
Biosensor
Electrochemistry
Contaminant
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Summary:This paper deals with the use of an electrochemical genosensor array for the rapid and simultaneous detection of different food-contaminating pathogenic bacteria. The method includes PCR amplification followed by analysis of the amplicons by hybridisation with toxin-specific oligonucleotide probes. A screen-printed array of four gold electrodes, modified using thiol-tethered oligonucleotide probes, was used. Unmodified PCR products were captured at the sensor interface via sandwich hybridisation with surface-tethered probes and biotinylated signaling probes. The resulting biotinylated hybrids were coupled with a streptavidin–alkaline phosphatase conjugate and then exposed to an α-naphthyl phosphate solution. Differential pulse voltammetry was finally used to detect the α-naphthol oxidation signal. Mixtures of DNA samples from different bacteria were detected at the nanomolar level without any cross-interference. The selectivity of the assay was also confirmed by the analysis of PCR products unrelated to the immobilised probes.
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content type line 23
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2006.06.001