The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae

Herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. H. rubrisubalbicans can also associate with various plants of economic interest in a non pathogenic manner. A 21 kb DNA region of the H. rubrisubalbicans genome contai...

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Published in:	BMC microbiology Vol. 12; no. 1; p. 98
Main Authors:	Schmidt, Maria Augusta, Balsanelli, Eduardo, Faoro, Hellison, Cruz, Leonardo M, Wassem, Roseli, de Baura, Valter A, Weiss, Vinícius, Yates, Marshall G, Madeira, Humberto M F, Pereira-Ferrari, Lilian, Fungaro, Maria H P, de Paula, Francine M, Pereira, Luiz F P, Vieira, Luiz G E, Olivares, Fábio L, Pedrosa, Fábio O, de Souza, Emanuel M, Monteiro, Rose A
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 06-06-2012 BioMed Central BMC
Subjects:	Bacteria, Phytopathogenic Bacterial Secretion Systems - genetics Bacteriology Colonization Disease susceptibility DNA DNA Transposable Elements DNA, Bacterial - chemistry DNA, Bacterial - genetics Economics Endophytes Endophytes - genetics Endophytes - pathogenicity Gene Deletion Genes Genetic aspects Genomes Genomics Herbaspirillum Herbaspirillum - genetics Herbaspirillum - pathogenicity Host-Pathogen Interactions Leaves Membrane Transport Proteins - genetics Microbiology Molecular Sequence Data Multigene Family Mutagenesis, Insertional Mutation Oryza sativa Pathogens Physiological aspects Plant diseases Plant Diseases - microbiology Plant-pathogen relationships Plasmids Poaceae Poaceae - microbiology Proteins Secretion Sequence Analysis, DNA stripe disease Sugarcane Vigna unguiculata Virulence Factors - genetics Zea mays Brazil
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Summary:	Herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. H. rubrisubalbicans can also associate with various plants of economic interest in a non pathogenic manner. A 21 kb DNA region of the H. rubrisubalbicans genome contains a cluster of 26 hrp/hrc genes encoding for the type three secretion system (T3SS) proteins. To investigate the contribution of T3SS to the plant-bacterial interaction process we generated mutant strains of H. rubrisubalbicans M1 carrying a Tn5 insertion in both the hrcN and hrpE genes. H. rubrisulbalbicans hrpE and hrcN mutant strains of the T3SS system failed to cause the mottled stripe disease in the sugarcane susceptible variety B-4362. These mutant strains also did not produce lesions on Vigna unguiculata leaves. Oryza sativa and Zea mays colonization experiments showed that mutations in hrpE and hrcN genes reduced the capacity of H. rubrisulbalbicans to colonize these plants, suggesting that hrpE and hrcN genes are involved in the endophytic colonization. Our results indicate that the T3SS of H. rubrisubalbicans is necessary for the development of the mottled stripe disease and endophytic colonization of rice.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1471-2180 1471-2180
DOI:	10.1186/1471-2180-12-98