Circulating endothelial cells and progenitors: potential biomarkers of renal cell carcinoma

OBJECTIVE To compare the levels of circulating endothelial cells (CECs) and progenitors (CEPs) between tumour‐bearing mice and healthy controls, in human renal cell carcinoma (RCC) xenograft models. The secondary objective was to correlate CEC and CEP levels with tumour variables such as tumour volu...

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Bibliographic Details
Published in:	BJU international Vol. 106; no. 7; pp. 1081 - 1087
Main Authors:	Namdarian, Benjamin, Tan, Kevin V.S., Fankhauser, Matthew J., Nguyen, Thanh T., Corcoran, Niall M., Costello, Anthony J., Hovens, Christopher M.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-10-2010 Wiley-Blackwell
Subjects:	angiogenesis Animals Biological and medical sciences biomarker Carcinoma, Renal Cell - blood supply Carcinoma, Renal Cell - pathology Cell Line, Tumor endothelial cells Endothelial Cells - metabolism Humans Kidney Neoplasms - blood supply Kidney Neoplasms - pathology Kidneys Male Medical sciences Mice Mice, SCID Neoplastic Cells, Circulating - pathology Neovascularization, Pathologic Nephrology. Urinary tract diseases Prognosis renal cell carcinoma Stem Cells - pathology Transplantation, Heterologous Tumors of the urinary system Kidney disease Endothelial cell Nephrology endothelial cells Urinary system disease Carcinoma Prognosis Stem cell Biological marker Biological indicator Malignant tumor biomarker Urology Angiogenesis renal cell carcinoma Kidney cancer Grawitz tumor Neovascularization Cancer Progenitor cell
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Summary:	OBJECTIVE To compare the levels of circulating endothelial cells (CECs) and progenitors (CEPs) between tumour‐bearing mice and healthy controls, in human renal cell carcinoma (RCC) xenograft models. The secondary objective was to correlate CEC and CEP levels with tumour variables such as tumour volume, weight and vascularity, indicators of disease severity. MATERIALS AND METHODS Two human RCC xenograft models were used. Tumour cells were inoculated either subcutaneously or beneath the renal subcapsule (orthotopic). Tumour dimensions were recorded and blood samples were taken throughout the experiment, as well as at the end of the experiment, upon which tumours were excised and prepared for histological examination. All blood samples were analysed by flow cytometry. RESULTS CEC and CEP levels were significantly elevated in tumour‐bearing mice compared with healthy controls. In particular, there was a divergence in CEC levels between RCC‐bearing mice and controls during early phases in disease, whereas CEP levels were only elevated towards the end. Additionally, CEC levels correlated with tumour variables such as tumour volume, when tumour volume was <200 mm3 and with tumour vascularity in certain models. CEP levels did not correlate significantly with most tumour variables examined. CONCLUSION In human RCC xenograft models, CEC levels showed promise as an adjuvant biomarker in evaluating disease burden. RESULTS from correlating CEC levels with tumour variables such as tumour volume, weight and vascularity suggested that CEC levels were a better prognostic indicator during early phases of tumour growth. CEP levels were elevated in tumour‐bearing mice compared with controls; however, enumerated numbers were small and require further validation in future studies.
Bibliography:	Equal first authors ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1464-4096 1464-410X
DOI:	10.1111/j.1464-410X.2010.09245.x