Enzyme-Catalyzed Polymerization Process: A Novel Approach to the Preparation of Polyaniline Colloidal Dispersions with an Immunomodulatory Effect

A green, nature-friendly synthesis of polyaniline colloidal particles based on enzyme-assisted oxidation of aniline with horseradish peroxidase and chitosan or poly(vinyl alcohol) as steric stabilizers was successfully employed. Physicochemical characterization revealed formation of particles conta...

Full description

Saved in:

Bibliographic Details
Published in:	Biomacromolecules Vol. 23; no. 8; pp. 3359 - 3370
Main Authors:	Jasenská, Daniela, Kašpárková, Věra, Vašíček, Ondřej, Münster, Lukáš, Minařík, Antonín, Káčerová, Simona, Korábková, Eva, Urbánková, Lucie, Vícha, Jan, Capáková, Zdenka, Falleta, Ermelinda, Della Pina, Cristina, Lehocký, Marián, Skopalová, Kateřina, Humpolíček, Petr
Format:	Journal Article
Language:	English
Published:	American Chemical Society 08-08-2022
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A green, nature-friendly synthesis of polyaniline colloidal particles based on enzyme-assisted oxidation of aniline with horseradish peroxidase and chitosan or poly(vinyl alcohol) as steric stabilizers was successfully employed. Physicochemical characterization revealed formation of particles containing the polyaniline emeraldine salt and demonstrated only a minor effect of polymer stabilizers on particle morphology. All tested colloidal particles showed in vitro antioxidation activity determined via scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. In vitro, they were able to reduce oxidative stress and inhibit the production of reactive oxygen species by neutrophils and inflammatory cytokines by macrophages. The anti-inflammatory effect observed was related to their antioxidant activity, especially in the case of neutrophils. The particles can thus be especially advantageous as active components of biomaterials modulating the early stages of inflammation. In addition to the immunomodulatory effect, the presence of intrinsically conducting polyaniline can impart cell-instructive properties to the particles. The approach to particle synthesis that we employedan original one using environmentally friendly and biocompatible horseradish peroxidaserepresents a smart way of preparing conducting particles with unique properties, which can be further modified by the stabilizers used.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1525-7797 1526-4602
DOI:	10.1021/acs.biomac.2c00371