Search Results - "Faley, Shannon L"

iPSC-Derived Brain Endothelium Exhibits Stable, Long-Term Barrier Function in Perfused Hydrogel Scaffolds by Faley, Shannon L., Neal, Emma H., Wang, Jason X., Bosworth, Allison M., Weber, Callie M., Balotin, Kylie M., Lippmann, Ethan S., Bellan, Leon M.

“…There is a profound need for functional, biomimetic in vitro tissue constructs of the human blood-brain barrier and neurovascular unit (NVU) to model diseases…”
Get full text

Advances in blood-brain barrier modeling in microphysiological systems highlight critical differences in opioid transport due to cortisol exposure by Brown, Jacquelyn A, Faley, Shannon L, Shi, Yajuan, Hillgren, Kathleen M, Sawada, Geri A, Baker, Thomas K, Wikswo, John P, Lippmann, Ethan S

“…The United States faces a national crisis involving opioid medications, where currently more than 130 people die every day. To combat this epidemic, a better…”
Get full text

Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit by Brown, Jacquelyn A, Faley, Shannon L, Judge, Monika, Ward, Patricia, Ihrie, Rebecca A, Carson, Robert, Armstrong, Laura, Sahin, Mustafa, Wikswo, John P, Ess, Kevin C, Neely, M Diana

“…Tuberous sclerosis complex (TSC) is a multi-system genetic disease that causes benign tumors in the brain and other vital organs. The most debilitating…”
Get full text

Modeling Neurovascular Disorders and Therapeutic Outcomes with Human-Induced Pluripotent Stem Cells by Bosworth, Allison M, Faley, Shannon L, Bellan, Leon M, Lippmann, Ethan S

“…The neurovascular unit (NVU) is composed of neurons, astrocytes, pericytes, and endothelial cells that form the blood-brain barrier (BBB). The NVU regulates…”
Get full text

Gravity-perfused airway-on-a-chip optimized for quantitative BSL-3 studies of SARS-CoV-2 infection: barrier permeability, cytokine production, immunohistochemistry, and viral load assays by Faley, Shannon L, Boghdeh, Niloufar A, Schaffer, David K, Spivey, Eric C, Alem, Farhang, Narayanan, Aarthi, Wikswo, John P, Brown, Jacquelyn A

“…Human microphysiological systems, such as organs on chips, are an emerging technology for modeling human physiology in a preclinical setting to understand the…”
Get more information

Microfluidic single cell arrays to interrogate signalling dynamics of individual, patient-derived hematopoietic stem cells by Faley, Shannon L, Copland, Mhairi, Wlodkowic, Donald, Kolch, Walter, Seale, Kevin T, Wikswo, John P, Cooper, Jonathan M

“…Stem cells hold great promise as a means of treating otherwise incurable, degenerative diseases due to their ability both to self-renew and differentiate…”
Get more information

SERS mapping of nanoparticle labels in single cells using a microfluidic chip by Syme, Christopher D, Sirimuthu, Narayana M S, Faley, Shannon L, Cooper, Jonathan M

“…We report for the first time the time-resolved mapping of intracellular nanoparticle labels from within living cells retained in a microstructured trap using…”
Get more information

Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis by Faley, Shannon L., Takahashi, Keiko, Crooke, Cornelia E., Beckham, Joshua T., Tomemori, Takuya, Shappell, Scott B., Jansen, E. Duco, Takahashi, Takamune

“…Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green…”
Get full text

A Customizable, Low-Cost Perfusion System for Sustaining Tissue Constructs by O'Grady, Brian J, Wang, Jason X, Faley, Shannon L, Balikov, Daniel A, Lippmann, Ethan S, Bellan, Leon M

“…The fabrication of engineered vascularized tissues and organs requiring sustained, controlled perfusion has been facilitated by the development of several pump…”
Get more information

Cell chip array for microfluidic proteomics enabling rapid in situ assessment of intracellular protein phosphorylation by Faley, Shannon L., Copland, Mhairi, Reboud, Julien, Cooper, Jonathan M.

“…We discuss the ability to perform fluorescent immunocytochemistry, following cell fixation, using a microfluidic array of primary, nonadherent, single CD 34 +…”
Get full text

Macro to nano: a simple method for transporting cultured cells from milliliter scale to nanoliter scale by Seale, Kevin T, Faley, Shannon L, Chamberlain, Jeff, Wikswo, Jr, John P

“…Microfluidic devices are well-suited for the study of metabolism and paracrine and autocrine signaling because they allow steady or intermittent perfusion of…”
Get more information

Robust fluidic connections to freestanding microfluidic hydrogels by Faley, Shannon L, Baer, Bradly B, Larsen, Taylor S H, Bellan, Leon M

“…Biomimetic scaffolds approaching physiological scale, whose size and large cellular load far exceed the limits of diffusion, require incorporation of a fluidic…”
Get full text

Quantum Dot Probes for Monitoring Dynamic Cellular Response: Reporters of T Cell Activation by Warnement, M.R., Faley, S.L., Wikswo, J.P., Rosenthal, S.J.

“…Antibody-conjugated quantum dots (QDs) have been used to map the expression dynamics of the cytokine receptor interleukin-2 receptor-alpha (IL-2Ralpha)…”
Get full text

Pachinko biology: Gambling on single cells by Faley, S.L., Copland, M., Wlodkovic, D., Kolch, W., Seale, K.T., Wikswo, J.P., Cooper, J.M.

“…While stem cells hold great promise as a means of treating otherwise incurable disease due to their characteristic ability to both self-renew and…”
Get full text