Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage

Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC...

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Published in:	Brazilian oral research Vol. 30; no. 1
Main Authors:	Gomes-Cornélio, Ana Lívia, Rodrigues, Elisandra Márcia, Mestieri, Leticia Boldrin, Falcoski, Thaís de Oliveira Rodrigues Sanzovo, Soares, Christiane Pienna, Guerreiro-Tanomaru, Juliane Maria, Rossa, Junior, Carlos, Tanomaru, Filho, Mário
Format:	Journal Article
Language:	English
Published:	Brazil Sociedade Brasileira de Pesquisa Odontológica - SBPqO 01-01-2016 Sociedade Brasileira de Pesquisa Odontológica
Subjects:	Aluminum Compounds - toxicity Analysis of Variance Apoptosis - drug effects Biocompatible Materials - toxicity Calcium Compounds Calcium Compounds - toxicity Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Comet Assay Cytotoxicity, Immunologic Dental Cements - toxicity Dentistry DENTISTRY, ORAL SURGERY & MEDICINE Drug Combinations Formazans Humans Materials Testing Mutagenicity Tests Necrosis - chemically induced Osteoblasts - drug effects Oxides - toxicity Reproducibility of Results Silicates - toxicity Tetrazolium Salts Calcium Compounds Mutagenicity Tests Cytotoxicity, Immunologic
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Summary:	Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni's posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1806-8324 1807-3107 1807-3107 1806-8324
DOI:	10.1590/1807-3107BOR-2016.VOL30.0048