Metabolic Profiling of Inga Species with Antitumor Activity

Metabolic Profiling of Inga Species with Antitumor Activity

This work evaluated the metabolic profiling of Inga species with antitumor potential. In addition, we described the antigenotoxicity of polyphenols isolated from I. laurina and a proteomic approach using HepG2 cells after treatment with these metabolites. The in vitro cytotoxic activity against HepG...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Vol. 27; no. 15; p. 4695
Main Authors: Lima, Nerilson M., Preet, Gagan, Marqui, Sara R., Falcoski, Thaís de O. R. S., Navegante, Geovana, Soares, Christiane P., Andrade, Teresinha de Jesus A. S., La Porta, Felipe A., Rakotondraie, Harinantenaina Liva R., Jaspars, Marcel, Silva, Dulce H. S.
Format: Journal Article
Language:English
Published: Basel MDPI AG 22-07-2022
MDPI
Subjects:
antigenotoxicity
Antitumor activity
Apoptosis
bioactive polyphenols
Bioassays
Biocompatibility
Biological activity
Cancer
Carotenoids
Chemical composition
Chromatography
Colon
Colon cancer
Comet assay
Cytotoxicity
Damage detection
DNA repair
Flavonoids
Fractionation
Fractions
Gene expression
Genotoxicity
Inga laurina
Metabolism
Metabolites
metabolomic
p-Hydroxybenzoic acid
Phenolic acids
Phenols
Polyphenols
Proteins
proteomic
Proteomics
reactive oxygen species
Seeds
Tumor cell lines
Tumors
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Summary:This work evaluated the metabolic profiling of Inga species with antitumor potential. In addition, we described the antigenotoxicity of polyphenols isolated from I. laurina and a proteomic approach using HepG2 cells after treatment with these metabolites. The in vitro cytotoxic activity against HepG2, HT-29 and T98G cancer cell lines was investigated. The assessment of genotoxic damage was carried out through the comet assay. The ethanolic extract from I. laurina seeds was subjected to bioassay-guided fractionation and the most active fractions were characterized. One bioactive fraction with high cytotoxicity against HT-29 human colon cancer cells (IC50 = 4.0 µg mL−1) was found, and it was characterized as a mixture of p-hydroxybenzoic acid and 4-vinyl-phenol. The I. edulis fruit peel (IC50 = 18.6 µg mL−1) and I. laurina seed (IC50 = 15.2 µg mL−1) extracts had cytotoxic activity against the cell line T98G, and its chemical composition showed a variety of phenolic acids. The chemical composition of this species indicated a wide variety of aromatic acids, flavonoids, tannins, and carotenoids. The high concentration (ranging from 5% to 30%) of these polyphenols in the bioactive extract may be responsible for the antitumor potential. Regarding the proteomic approach, we detected proteins directly related to the elimination of ROS, DNA repair, expression of tumor proteins, and apoptosis.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27154695