Characterization of scrapie infection in mouse neuroblastoma cells

U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840, U.S.A. A mouse neuroblastoma cell line was success...

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Bibliographic Details
Published in:	Journal of general virology Vol. 68; no. 5; pp. 1391 - 1399
Main Authors:	Race, R.E, Fadness, L.H, Chesebro, B
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-05-1987 Society for General Microbiology
Subjects:	Animals Biological and medical sciences brain Brain - microbiology cell culture Cell Line characterization Cricetinae Fundamental and applied biological sciences. Psychology infection Mice Microbiology neuroblastoma Neuroblastoma - pathology Prions - isolation & purification Prions - physiology PrPSc proteins replication Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Species Specificity Spleen - microbiology Virology Virus Cultivation Virus Vertebrata Mammalia Cell line Virus multiplication Mouse Rodentia Neuroblastoma Scrapie agent
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Summary:	U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, Hamilton, Montana 59840, U.S.A. A mouse neuroblastoma cell line was successfully infected with scrapie agent. Agent derived from infected mouse brain or spleen infected cultures. However, agent from infected hamsters did not infect mouse cell cultures, suggesting that species specificity influenced the infection process in vitro . Positive cultures supported scrapie replication for as many as 47 passages in vitro . Agent was shown to be cell-associated and between 631 and 7943 unselected culture cells constituted 1 mouse LD 50 . However, fluctuation analysis indicated that only one of 144 cells in unselected cultures was actually infected. Thus, agent was confined to a small percentage of cells and only 4.4 to 55.1 positive cells were needed to confer a mouse LD 50 . Keywords: scrapie, neuroblastoma cells, limiting dilution analysis Received 10 November 1986; accepted 23 January 1987.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0022-1317 1465-2099
DOI:	10.1099/0022-1317-68-5-1391