Search Results - "FORRER, Patrik"

Accelerated dissociation of IgE-FcεRI complexes by disruptive inhibitors actively desensitizes allergic effector cells by Eggel, Alexander, PhD, Baravalle, Günther, PhD, Hobi, Gabriel, BSc, Kim, Beomkyu, PhD, Buschor, Patrick, MSc, Forrer, Patrik, PhD, Shin, Jeoung-Sook, PhD, Vogel, Monique, PhD, Stadler, Beda M., PhD, Dahinden, Clemens A., MD, Jardetzky, Theodore S., PhD

“…Background The remarkably stable interaction of IgE with its high-affinity receptor FcεRI on basophils and mast cells is critical for the induction of allergic…”
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Half-life extension using serum albumin-binding DARPin® domains by Steiner, Daniel, Merz, Frieder W, Sonderegger, Ivo, Gulotti-Georgieva, Maya, Villemagne, Denis, Phillips, Douglas J, Forrer, Patrik, Stumpp, Michael T, Zitt, Christof, Binz, H Kaspar

“…Abstract A long systemic half-life is key for therapeutic proteins. To that end we have generated serum albumin-binding designed ankyrin repeat domains. These…”
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High-affinity binders selected from designed ankyrin repeat protein libraries by Kohl, Andreas, Forrer, Patrik, Briand, Christophe, Stumpp, Michael T, Binz, H Kaspar, Amstutz, Patrick, Grütter, Markus G, Plückthun, Andreas

“…We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. Using a consensus design strategy, we…”
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Designed to Be Stable: Crystal Structure of a Consensus Ankyrin Repeat Protein by Kohl, Andreas, Binz, H. Kaspar, Forrer, Patrik, Stumpp, Michael T., Plückthun, Andreas, Grütter, Markus G.

“…Ankyrin repeat (AR) proteins mediate innumerable protein-protein interactions in virtually all phyla. This finding suggested the use of AR proteins as designed…”
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Intracellular Kinase Inhibitors Selected from Combinatorial Libraries of Designed Ankyrin Repeat Proteins by Amstutz, Patrick, Binz, H. Kaspar, Parizek, Petra, Stumpp, Michael T., Kohl, Andreas, Grütter, Markus G., Forrer, Patrik, Plückthun, Andreas

“…The specific intracellular inhibition of protein activity at the protein level allows the determination of protein function in the cellular context. We…”
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Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display by STEINER, Daniel, FORRER, Patrik, STUMPP, Michael T, PLÜCKTHUN, Andreas

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Isolation of Intracellular Proteinase Inhibitors Derived from Designed Ankyrin Repeat Proteins by Genetic Screening by Kawe, Martin, Forrer, Patrik, Amstutz, Patrick, Plückthun, Andreas

“…The specific intracellular inhibition of protein activity at the protein level is a highly valuable tool for the validation or modulation of cellular…”
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Designing Repeat Proteins: Modular Leucine-rich Repeat Protein Libraries Based on the Mammalian Ribonuclease Inhibitor Family by Stumpp, Michael T., Forrer, Patrik, Binz, H.Kaspar, Plückthun, Andreas

“…We present a novel approach to design repeat proteins of the leucine-rich repeat (LRR) family for the generation of libraries of intracellular binding…”
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Consensus Design of Repeat Proteins by Forrer, Patrik, Binz, H. Kaspar, Stumpp, Michael T, Plückthun, Andreas

“…Consensus design is a valuable protein-engineering method that is based on statistical information derived from sequence alignments of homologous proteins…”
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High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage Lambda head protein D by Forrer, Patrik, Jaussi, Rolf

“…The bacteriophage Lambda head protein D (gpD) is a small major capsid protein (110 aa; 11.6 kDa; pI=5.68, devoid of cysteine residues) that is essential for…”
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Kinetic Stability and Crystal Structure of the Viral Capsid Protein SHP by Forrer, Patrik, Chang, Changsoo, Ott, David, Wlodawer, Alexander, Plückthun, Andreas

“…SHP, the capsid-stabilizing protein of lambdoid phage 21, is highly resistant against denaturant-induced unfolding. We demonstrate that this high functional…”
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Efficient Selection of DARPins with Sub-nanomolar Affinities using SRP Phage Display by Steiner, Daniel, Forrer, Patrik, Plückthun, Andreas

“…There is an ever-increasing demand to select specific, high-affinity binding molecules against targets of biomedical interest. The success of such selections…”
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Thermostable designed ankyrin repeat proteins (DARPins) as building blocks for innovative drugs by Schilling, Johannes, Jost, Christian, Ilie, Ioana Mariuca, Schnabl, Joachim, Buechi, Oralea, Eapen, Rohan S., Truffer, Rafaela, Caflisch, Amedeo, Forrer, Patrik

“…Designed ankyrin repeat proteins (DARPins) are antibody mimetics with high and mostly unexplored potential in drug development. By using in silico analysis and…”
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Novel fold and capsid-binding properties of the λ-phage display platform protein gpD by Plückthun, Andreas, Yang, Fan, Forrer, Patrik, Dauter, Zbigniew, Conway, James F, Cheng, Naiqian, Cerritelli, Mario E, Steven, Alasdair C, Wlodawer, Alexander

“…The crystal structure of gpD, the capsid-stabilizing protein of bacteriophage lambda, was solved at 1.1 A resolution. Data were obtained from twinned crystals…”
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PDZK1: I. A major scaffolder in brush borders of proximal tubular cells11See Editorial by Moe, p. 1916 by Gisler, Serge M., Pribanic, Sandra, Bacic, Desa, Forrer, Patrik, Gantenbein, Andrea, Sabourin, Luc A., Tsuji, Akira, Zhao, Zhuo-Shen, Manser, Edward, Biber, Jürg, Murer, Heini

“…PDZK1: I. A major scaffolder in brush borders of proximal tubular cells. In proximal tubular cells, PDZK1 (NaPi-Cap1) has been implicated in apical expression…”
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Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display by Plückthun, Andreas, Steiner, Daniel, Forrer, Patrik, Stumpp, Michael T

“…Even proteins that fold well in bacteria are frequently displayed poorly on filamentous phages. Low protein presentation on phage might be caused by premature…”
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Designing Repeat Proteins: Well-expressed, Soluble and Stable Proteins from Combinatorial Libraries of Consensus Ankyrin Repeat Proteins by Binz, H.Kaspar, Stumpp, Michael T, Forrer, Patrik, Amstutz, Patrick, Plückthun, Andreas

“…We describe an efficient way to generate combinatorial libraries of stable, soluble and well-expressed ankyrin repeat (AR) proteins. Using a combination of…”
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Highly potent VEGF-A-antagonistic DARPins as anti-angiogenic agents for topical and intravitreal applications by Stahl, Andreas, Stumpp, Michael T., Schlegel, Anja, Ekawardhani, Savira, Lehrling, Christina, Martin, Gottfried, Gulotti-Georgieva, Maya, Villemagne, Denis, Forrer, Patrik, Agostini, Hansjürgen T., Binz, H. Kaspar

“…The next-generation ophthalmic anti-VEGF therapeutics must aim at being superior to the currently available agents with regard to potency and improved drug…”
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Her2-specific Multivalent Adapters Confer Designed Tropism to Adenovirus for Gene Targeting by Dreier, Birgit, Mikheeva, Galina, Belousova, Natalya, Parizek, Petra, Boczek, Edgar, Jelesarov, Ilian, Forrer, Patrik, Plückthun, Andreas, Krasnykh, Victor

“…Adenoviruses (Ads) hold great promise as gene vectors for diagnostic or therapeutic applications. The native tropism of Ads must be modified to achieve disease…”
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PDZK1: I. A major scaffolder in brush borders of proximal tubular cells by GISLER, Serge M, PRIBANIC, Sandra, MURER, Heini, BACIC, Desa, FORRER, Patrik, GANTENBEIN, Andrea, SABOURIN, Luc A, TSUJI, Akira, ZHAO, Zhuo-Shen, MANSER, Edward, BIBER, Jürg

“…In proximal tubular cells, PDZK1 (NaPi-Cap1) has been implicated in apical expression of the Na+-dependent phosphate cotransporter (NaPi-IIa) via interaction…”
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