Telomerase activity in pulmonary neuroendocrine tumors: Correlation with histologic subtype (MS-0060)

Telomerase activity in pulmonary neuroendocrine tumors: Correlation with histologic subtype (MS-0060)

The authors measured telomerase activity using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay method in 13 neuroendocrine pulmonary neoplasms and in non-neoplastic frozen lung samples from the same patients. These cases belonged to the complete neuroendocrine neoplasti...

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Bibliographic Details
Published in:The American journal of surgical pathology Vol. 24; no. 3; pp. 417 - 421
Main Authors: GOMEZ-ROMAN, J. J, FONTALBA ROMERO, A, SANCHEZ CASTRO, L, HERNANDEZ, E. NIETO, FERNANDEZ-LUNA, J. L, VAL-BERNAL, J. F
Format: Journal Article
Language:English
Published: Hagerstown, MD Lippincott Williams & Wilkins 01-03-2000
Subjects:
Adult
Aged
Biological and medical sciences
Female
Humans
Lung Neoplasms - enzymology
Lung Neoplasms - pathology
Male
Medical sciences
Middle Aged
Neuroendocrine Tumors - enzymology
Neuroendocrine Tumors - pathology
Pneumology
Telomerase - metabolism
Tumors of the respiratory system and mediastinum
Human
Lung disease
Correlation
Prognosis
Respiratory disease
Pathogenesis
Lung
Histological type
Activity
Neuroendocrine tumor
ELISA assay
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Summary:The authors measured telomerase activity using the telomeric repeat amplification protocol-enzyme-linked immunosorbent assay method in 13 neuroendocrine pulmonary neoplasms and in non-neoplastic frozen lung samples from the same patients. These cases belonged to the complete neuroendocrine neoplastic spectrum: four typical carcinoids, three atypical carcinoids, four large cell neuroendocrine lung carcinomas, and two small cell lung carcinomas. The authors performed the same assay for 52 non-neoplastic lung tissues from the surgical files in their department (negative controls). They verified the presence (or absence) of neoplastic tissue in every case by looking at one frozen section done in the same tissue used for telomerase assay. The telomerase activity level in non-neoplastic tissues (mean, 182 A450nm U) was similar to that obtained in the typical carcinoids (mean, 104.5 A450nm U). All neuroendocrine tumors but the typical carcinoids showed high levels of telomerase activity (mean, 1,750.8 A450nm U). According to the telomerase hypothesis, typical carcinoid cells are mortal pre-M2 stage cells, but atypical carcinoid, large cell neuroendocrine lung carcinoma, and small cell lung carcinoma cells are immortal post-M2 stage cells. This finding may be of important prognostic significance in these kinds of tumors. Measurement of enzyme activity with a good morphologic control could be necessary in telomerase activity assay.
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ISSN:0147-5185
1532-0979
DOI:10.1097/00000478-200003000-00011