Search Results - "FAY, F. S"

Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses by RIZZUTO, R, PINTON, P, CARRINGTON, W, FAY, F. S, FOGARTY, K. E, LIFSHITZ, L. M, TUFT, R. A, POZZAN, T

“…The spatial relation between mitochondria and endoplasmic reticulum (ER) in living HeLa cells was analyzed at high resolution in three dimensions with two…”
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Visualization of Single RNA Transcripts in Situ by Femino, Andrea M., Fay, Fredric S., Fogarty, Kevin, Singer, Robert H.

“…Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes…”
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Disruption of PDGF Receptor Trafficking by Mutation of Its PI-3 Kinase Binding Sites by Joly, Marguerite, Kazlauskas, Andrius, Fay, Fredric S., Corvera, Silvia

“…Human platelet-derived growth factor receptors (PDGFRs) expressed in human Hep G2 cells internalized and concentrated in a juxtanuclear region near the Golgi…”
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Superresolution Three-Dimensional Images of Fluorescence in Cells with Minimal Light Exposure by Carrington, Walter A., Lynch, Ronald M., Edwin D. W. Moore, Isenberg, Gerrit, Fogarty, Kevin E., Fay, Fredric S.

“…Fluorescent probes offer insight into the highly localized and rapid molecular events that underlie cell function. However, methods are required that can…”
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Serum-Induced Translocation of Mitogen-Activated Protein Kinase to the Cell Surface Ruffling Membrane and the Nucleus by Gonzalez, Fernando A., Seth, Alpna, Raden, David L., Bowman, Douglas S., Fay, Fredric S., Davis, Roger J.

“…The mitogen-activated protein (MAP) kinase signal transduction pathway represents an important mechanism by which growth factors regulate cell function…”
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Detection of changes in near-membrane Ca2+ concentration using a novel membrane-associated Ca2+ indicator by ETTER, E. F, KUHN, M. A, FAY, F. S

“…A Ca2+ indicator has been synthesized and characterized which can be used to monitor rapid changes in the free Ca2+ concentration ([Ca2+]) immediately adjacent…”
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A Three-Dimensional View of Precursor Messenger RNA Metabolism Within the Mammalian Nucleus by Carter, Kenneth C., Bowman, Douglas, Carrington, Walter, Fogarty, Kevin, McNeil, John A., Fay, Fredric S., Lawrence, Jeanne Bentley

“…A quantitative three-dimensional analysis of nuclear components involved in precursor messenger RNA metabolism was performed with a combination of fluorescence…”
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Physiological cytosolic Ca2+ transients evoke concurrent mitochondrial depolarizations by Loew, L M, Carrington, W, Tuft, R A, Fay, F S

“…Calcium, a ubiquitous second messenger, stimulates the activity of several mitochondrial dehydrogenases. This has led to the suggestion that the same messenger…”
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Near-Membrane [Ca2+] Transients Resolved Using the Ca2+ Indicator FFP18 by Etter, Elaine F., Minta, Akwasi, Poenie, Martin, Fay, Fredric S.

“…Ca2+-sensitive processes at cell membranes involved in contraction, secretion, and neurotransmitter release are activated in situ or in vitro by Ca2+…”
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Photobleaching of fura-2 and its effect on determination of calcium concentrations by Becker, P L, Fay, F S

“…This study was performed to determine the effect of photobleaching on the spectral properties of the calcium-sensitive fluorescent dye fura-2. Fura-2, whether…”
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Coupling of the Na+/Ca2+ exchanger, Na+/K+ pump and sarcoplasmic reticulum in smooth muscle by MOORE, E. D. W, ETTER, E. F, PHILIPSON, K. D, CARRINGTON, W. A, FOGARTY, K. E, LIFSHITZ, L. M, FAY, F. S

“…The Na+/Ca2+ exchanger, driven by a transmembrane Na+ gradient, plays a key role in regulating Ca2+ concentration in many cells. Although the exchanger…”
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Imaging in five dimensions: time-dependent membrane potentials in individual mitochondria by Loew, L.M., Tuft, R.A., Carrington, W., Fay, F.S.

“…Because of its importance in the chemiosmotic theory, mitochondrial membrane potential has been the object of many investigations. Significantly, however,…”
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Intracellular calibration of the fluorescent calcium indicator Fura-2 by Williams, D A, Fay, F S

“…We present the techniques we have used and the problems we have encountered in our laboratories in the in vivo calibration of the fluorescent Ca2(+)-indicator…”
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Ca2+ movement in smooth muscle cells studied with one- and two-dimensional diffusion models by Kargacin, G., Fay, F.S.

“…Although many of the processes involved in the regulation of Ca2+ in smooth muscle have been studied separately, it is still not well known how they are…”
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Calcium signalling in sarcoplasmic reticulum, cytoplasm and mitochondria during activation of rabbit aorta myocytes by Gurney, A.M., Drummond, R.M., Fay, F.S.

“…This study investigated the relationship between cytoplasmic, mitochondrial, and sarcoplasmic reticulum (SR) [Ca2+] in rabbit aorta smooth muscle cells,…”
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The temporal profile of calcium transients in voltage clamped gastric myocytes from Bufo marinus by McGeown, J G, Drummond, R M, McCarron, J G, Fay, F S

“…1. Decay in intracellular calcium concentration ([Ca2+]i) was recorded following step depolarizations in voltage clamped gastric myocytes from Bufo marinus. 2…”
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Calcium gradients in single smooth muscle cells revealed by the digital imaging microscope using Fura-2 by Williams, David A, Fogarty, Kevin E, Tsien, Roger Y, Fay, Fredric S

“…Calcium is believed to control a variety of cellular processes, often with a high degree of spatial and temporal precision. For a cell to use Ca2+ in this…”
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Antibodies Probe for Folded Monomeric Myosin in Relaxed and Contracted Smooth Muscle by Horowitz, Arie, Trybus, Kathleen M., Bowman, Douglas S., Fay, Fredric S.

“…Regulatory light chain phosphorylation is required for assembly of smooth and non-muscle myosins in vitro, but its effect on polymerization within the cell is…”
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Calcium Sparks in Vascular Smooth Muscle: Relaxation Regulators by Fay, Fredric S.

“…Calcium “sparks” are small, intense bursts of calcium that occur in cells from cardiac muscle, skeletal muscle, and, as shown in a paper in this issue of…”
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Calcium-calmodulin-dependent mechanisms accelerate calcium decay in gastric myocytes from Bufo marinus by McGeown, J. G., McCarron, J. G., Drummond, R. M., Fay, F. S.

“…[Ca 2+ ] i was recorded in voltage-clamped gastric myocytes from Bufo marinus . Repolarization to -110 mV following a 300 ms depolarization to +10 mV led to…”
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