MONITORING OF THE H275Y MUTATION IN PANDEMIC INFLUENZA A(H1N1) 2009 STRAINS ISOLATED IN MALAYSIA

The 2009 pandemic influenza A(H1N1) infection in Malaysia was first reported in May 2009 and oseltamivir was advocated for confirmed cases in postexposure prophylaxis. However, there are cases of oseltamivir-resistance reported among H1N1-positive patients in other countries. Resistance is due to su...

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Published in:	Southeast Asian journal of tropical medicine and public health Vol. 42; no. 1; pp. 100 - 104
Main Authors:	SUPPIAH, Jeyanthi, MOHD APANDI YUSOF, KHAIRUL AZUAN OTHMAN, SARASWATHY, T. S, THAYAN, Ravindran, FAUZIAH MD KASIM, MURAD, Shahnaz, SAAT, Zainah
Format:	Journal Article
Language:	English
Published:	Bangkok SEAMO, Regional Tropical Medicine and Public Health Network 2011 Central Coordinating Board, SEAMEO-TROPMED Project
Subjects:	Amino Acid Substitution Antiviral Agents - therapeutic use Biological and medical sciences Disease Outbreaks Drug Resistance, Viral General aspects Human viral diseases Humans Infectious diseases Influenza A Virus, H1N1 Subtype - enzymology Influenza A Virus, H1N1 Subtype - genetics Influenza, Human - drug therapy Influenza, Human - epidemiology Influenza, Human - virology Malaysia - epidemiology Medical sciences Mutation, Missense Neuraminidase - genetics Oseltamivir - therapeutic use Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Viral diseases Viral diseases of the respiratory system and ent viral diseases Viral Proteins - genetics Asia Malaysia Infection Surveillance Respiratory disease H1N1 influenza Viral disease Influenza A (H1N1) Tropical medicine Mutation
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Summary:	The 2009 pandemic influenza A(H1N1) infection in Malaysia was first reported in May 2009 and oseltamivir was advocated for confirmed cases in postexposure prophylaxis. However, there are cases of oseltamivir-resistance reported among H1N1-positive patients in other countries. Resistance is due to substitution of histidine by tyrosine at residue 275 (H275Y) of neuraminidase (NA). In this study, we have employed Sanger sequencing method to investigate the occurrence of mutations in NA segments of 67 pandemic 2009 A(H1N1) viral isolates from Malaysian patients that could lead to probable oseltamivir resistance. The sequencing analysis did not yield mutation at residue 275 for all 67 isolates indicating that our viral isolates belong to the wild type and do not confer resistance to oseltamivir.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0125-1562