Valorization of agro-industrial residues for pectinase production by Aspergillus aculeatus: Application in cashew fruit juice clarification
•Fungal isolate identified as Aspergillus aculeatus demonstrated pectinolytic activity.•The isolate showed maximum pectinase production in mango peel amended media.•Pectinase optimum activity was obtained at pH (5.5) and temperature (40 °C).•The enzyme hydrolyzed pectin in cashew juice; reducing clo...
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| Published in: | Cleaner and Circular Bioeconomy Vol. 4; p. 100038 |
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| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier Ltd
01-04-2023
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | •Fungal isolate identified as Aspergillus aculeatus demonstrated pectinolytic activity.•The isolate showed maximum pectinase production in mango peel amended media.•Pectinase optimum activity was obtained at pH (5.5) and temperature (40 °C).•The enzyme hydrolyzed pectin in cashew juice; reducing cloudiness and viscosity.
Lignocellulosic waste materials are exorbitantly generated from agroindustrial processing, which contribute significantly to the carbon footprint. Production of important microbial enzymes using these non-food agro-residues could subsidize the cost of biotechnological processes and also ensure sustainability. Consequently, fungal isolates were recovered from pectin-rich soil samples, and the most potent isolate evaluated for pectinase production in media constituted with agro-industrial wastes. The fungal isolate coded as NEJC showed maximum pectinase production on day 8 (262.5 ± 3.53 U/L) of the fermentation process. Among the agro-residues evaluated, the isolate showed the highest pectinase titer (1360 ± 254.55 U/L) when mango peel served as the sole carbon source. The fungal isolate was identified as Aspergillus aculeatus NEJC (accession no. MT951632). Furthermore, the pectinase optimum activity was obtained at pH and temperature of 5.5 and 40°C, respectively. In addition, the enzyme exhibited a significant cashew juice clarification capacity by reducing the cloudiness with 44% and 56% viscosity reduction after 30 min and 60 min of pre-incubation, respectively. The findings of the study highlight the significance of the agro-wastes for sustainable production of fungal pectinase. In addition, the properties of the fungal pectinase underpinned its application prospects in bioprocess technology. |
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| ISSN: | 2772-8013 2772-8013 |
| DOI: | 10.1016/j.clcb.2023.100038 |