Evaluation of antimicrobial resistance, biofilm forming potential, and the presence of biofilm-related genes among clinical isolates of Pseudomonas aeruginosa

Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and...

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Published in:	BMC research notes Vol. 13; no. 1; p. 27
Main Authors:	Kamali, Esmat, Jamali, Ailar, Ardebili, Abdollah, Ezadi, Freshteh, Mohebbi, Alireza
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 10-01-2020 BioMed Central BMC
Subjects:	Amikacin Aminoglycoside antibiotics Antibacterial agents Antibiotics Antimicrobial agents Antimicrobial resistance Beta lactamases Biofilm formation Biofilm genes Biofilms Biosynthesis Carbapenems Ceftazidime Clinical isolates Deoxyribonucleic acid DNA Drug resistance Drug therapy Evaluation Fluoroquinolones Genes Genetic aspects Imipenem Infection Infections Laboratories Lactams Levofloxacin Medical research Meropenem Microbial drug resistance Microbial mats Multidrug resistance Nosocomial infection Phenotypes Piperacillin Pseudomonas aeruginosa Pseudomonas aeruginosa infections Pseudomonas infections Research Note Tazobactam Virulence factors β-Lactam antibiotics Iran United States > US Germany Pseudomonas aeruginosa Antimicrobial resistance Biofilm genes Biofilm formation
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Summary:	Pseudomonas aeruginosa is known as a leading cause of nosocomial infections worldwide. Antimicrobial resistance and biofilm production, as two main virulence factors of P. aeruginosa, are responsible for the persistence of prolonged infections. In this study, antimicrobial susceptibility pattern and phenotypic and genotypic characteristics of biofilm of P. aeruginosa were investigated. A total of 80 clinical P. aeruginosa isolates were obtained. Isolates showed resistance to all antibiotics with a rate from 12.5% (n = 10) against amikacin and piperacillin/tazobactam to 23.75% (n = 19) to levofloxacin. Multidrug-resistant P. aeruginosa accounted for 20% (n = 16). 83.75% (n = 67) of isolates showed biofilm phenotype. All three biofilm-related genes were found simultaneously in 87.5% (n = 70) of P. aeruginosa and 13.5% (n = 10) of the isolates had none of the genes tested. From the results of the present study, combination therapy including an anti-pseudomonal beta-lactam (piperacillin/tazobactam or ceftazidime) and an aminoglycoside or carbapenems (imipenem, meropenem) with fluoroquinolones in conjunction with an aminoglycoside can be used against Pseudomonas infections. However, reasonable antimicrobial use and high standards of infection prevention and control are essential to prevent further development of antimicrobial resistance. Combination strategies based on the proper anti-pseudomonal antibiotics along with anti-biofilm agents can also be selected to eradicate biofilm-associated infections.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1756-0500 1756-0500
DOI:	10.1186/s13104-020-4890-z