Molecular detection of Coxiella burnetii in horse sera in Iran

Molecular detection of Coxiella burnetii in horse sera in Iran

•In this manuscript, we detect the Coxiella burnetii DNA by Molecular Method in horse sera for the first time in Iran.•Coxiella burnetii is a high pathogenic organism that can cause Q fever with 1–10 Numbers of bacteria.•Important as zoonotic disease, its circulation dynamics in and through horses a...

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Published in:Comparative immunology, microbiology and infectious diseases Vol. 72; p. 101521
Main Authors: Khademi, P, Ownagh, A, Ataei, B, Kazemnia, A, Eydi, J, Khalili, M, M, Mahzounieh, Mardani, K
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-10-2020
Subjects:
Animals
Coxiella burnetii
Coxiella burnetii - genetics
Coxiella burnetii - isolation & purification
Female
Golestan province
Horse Diseases - diagnosis
Horse Diseases - epidemiology
Horse sera
Horses
Iran
Iran - epidemiology
Male
Nested-PCR
Polymerase Chain Reaction - veterinary
Prevalence
Q Fever - diagnosis
Q Fever - epidemiology
Q Fever - veterinary
Iran
Golestan province
Nested-PCR
Iran
Horse sera
Coxiella burnetii
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Summary:•In this manuscript, we detect the Coxiella burnetii DNA by Molecular Method in horse sera for the first time in Iran.•Coxiella burnetii is a high pathogenic organism that can cause Q fever with 1–10 Numbers of bacteria.•Important as zoonotic disease, its circulation dynamics in and through horses are still unclear. Coxiella burnetii is a zoonotic bacterium that can infect a wide range of animals including horses. However, its circulation dynamics in and through horses are still unclear. The aim of this study was to evaluate prevalence of C. burnetii and its genomic characteristics in horse sera samples in the North of Iran (Golestan Province). The samples were collected in 2018 and the age, sex, and breed of each animal were recorded. Nested-PCR was used to detect C. burnetii based on the presence of the transposable gene IS1111. The results showed that 7.50 % (P < 0.05; 95 % CI: 0.5 %–0.12 %) of the examined sera samples were positive for C. burnetii. Based on the resuls, prevalence of C. burnetii in the age groupof < Years 1–5 (p-value <0.05, 95 % CI: 1 %–8 %) was less than the age group of >6 years old (p-value <0.05, 95 %, CI: 7 %–19.8 %). In previous studies, it was concluded that the horses' population in Golestan Province should be considered as an important factor in the epidemiology of Q fever and consequently in public health. Further studies should be implemented to evaluate if horses may be relevant indicators of zoonotic risk in urban and suburban endemic areas.
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ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2020.101521