Search Results - "Evers, Toon H."

How Antibody Surface Coverage on Nanoparticles Determines the Activity and Kinetics of Antigen Capturing for Biosensing by Saha, Bedabrata, Evers, Toon H, Prins, Menno W. J

“…The antigen-capturing activity of antibody-coated nanoparticles is very important for affinity-based bioanalytical tools. In this paper, a comprehensive study…”
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Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Linkers by Evers, Toon H, van Dongen, Elisabeth M. W. M, Faesen, Alex C, Meijer, E. W, Merkx, Maarten

“…The fusion of different protein domains via peptide linkers is a powerful, modular approach to obtain proteins with new functions. A detailed understanding of…”
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Rapid, high sensitivity, point-of-care test for cardiac troponin based on optomagnetic biosensor by Dittmer, Wendy U., Evers, Toon H., Hardeman, Willie M., Huijnen, Willeke, Kamps, Rick, de Kievit, Peggy, Neijzen, Jaap H.M., Nieuwenhuis, Jeroen H., Sijbers, Mara J.J., Dekkers, Dave W.C., Hefti, Marco H., Martens, Mike F.W.C.

“…We present a prototype handheld device based on a newly developed optomagnetic technology for the sensitive detection of cardiac troponin I (cTnI) in a…”
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Variation of Linker Length in Ratiometric Fluorescent Sensor Proteins Allows Rational Tuning of Zn(II) Affinity in the Picomolar to Femtomolar Range by van Dongen, Elisabeth M. W. M, Evers, Toon H, Dekkers, Linda M, Meijer, E. W, Klomp, Leo W. J, Merkx, Maarten

“…Ratiometric fluorescent sensor proteins with a very high and tunable affinity for Zn(II) were created by connecting two fluorescently labeled metal binding…”
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Ratiometric Detection of Zn(II) Using Chelating Fluorescent Protein Chimeras by Evers, Toon H., Appelhof, Marieke A.M., de Graaf-Heuvelmans, Peggy T.H.M., Meijer, E.W., Merkx, Maarten

“…Fluorescent indicators for the real-time imaging of small molecules or metal ions in living cells are invaluable tools for understanding their physiological…”
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Enhanced Sensitivity of FRET-Based Protease Sensors by Redesign of the GFP Dimerization Interface by Vinkenborg, Jan L., Evers, Toon H., Reulen, Sanne W. A., Meijer, E. W., Merkx, Maarten

“…Close encounters. Sensor proteins based on fluorescence resonance energy transfer (FRET) often display a modest change in emission ratio upon activation. Here,…”
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The influence of covalent immobilization conditions on antibody accessibility on nanoparticles by Saha, Bedabrata, Songe, Pål, Evers, Toon H, Prins, Menno W J

“…The accessibility of particle-coupled antibodies is important for many analytical applications, but comprehensive data on parameters controlling the…”
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Electron Transfer and Ligand Binding to Cytochrome c‘ Immobilized on Self-Assembled Monolayers by de Groot, Matheus T, Evers, Toon H, Merkx, Maarten, Koper, Marc T. M

“…We have successfully immobilized Allochromatium vinosum cytochrome c‘ on carboxylic acid-terminated thiol monolayers on gold and have investigated its…”
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Successful recombinant production of Allochromatium vinosum cytochrome c′ requires coexpression of cmm genes in heme-rich Escherichia coli JCB712 by Evers, Toon H., Merkx, Maarten

“…Cytochrome c′ from the purple photosynthetic bacterium Allochromatium vinosum (CCP) displays a unique, reversible dimer-to-monomer transition upon binding of…”
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Ligand-induced monomerization of Allochromatium vinosum cytochrome c' studied using native mass spectrometry and fluorescence resonance energy transfer by Evers, Toon H, van Dongen, Joost L J, Meijer, E W, Merkx, Maarten

“…Cytochrome c' from Allochromatium vinosum is an attractive model protein to study ligand-induced conformational changes. This homodimeric protein dissociates…”
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Influence of dsDNA fragment length on particle binding in an evanescent field biosensing system by Koets, Marjo, van Ommering, Kim, Wang, Liqin, Testori, Emilie, Evers, Toon H, Prins, Menno W J

“…Particle labels are widely used in affinity-based biosensing due to the high detection signal per label, the high stability, and the convenient…”
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His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor by Evers, Toon H., Appelhof, Marieke A.M., Meijer, E.W., Merkx, Maarten

“…Fluorescent indicators that allow real-time imaging of Zn(II) in living cells are invaluable tools for understanding Zn(II) homeostasis. Genetically encoded…”
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Protein biomarker enrichment by biomarker antibody complex elution for immunoassay biosensing by Sabatte, Gwenola, Feitsma, Harma, Evers, Toon H., Prins, Menno W.J.

“…It is very challenging to perform sample enrichment for protein biomarkers because proteins can easily change conformation and denature. In this paper we…”
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Structural Requirements of the Fructan-Lipid Interaction by Vereyken, Ingrid J., Albert van Kuik, J., Evers, Toon H., Rijken, Pieter J., de Kruijff, Ben

“…Fructans are a group of fructose-based oligo- and polysaccharides. They are proposed to be involved in membrane protection of plants during dehydration. In…”
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Protein biomarker enrichment by biomarker antibody complex elution for immunoassay biosensing by SABATTE, Gwenola, FEITSMA, Harma, EVERS, Toon H, PRINS, Menno W. J

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Cover Picture: Enhanced Sensitivity of FRET-Based Protease Sensors by Redesign of the GFP Dimerization Interface (ChemBioChem 10/2007) by Vinkenborg, Jan L., Evers, Toon H., Reulen, Sanne W. A., Meijer, E. W., Merkx, Maarten

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