Pathogenetic Effects of Bacteria of the Genus Desulfovibrio. Experimental Study. Part One: Iron Metabolism

The aim of the work was to study specific systemic effect of sulfate-reducing bacteria of the genus Desulfovibrio on organism systems through creating an experimental model of intragastric bacterial invasion against the background of dysbiotic conditions of rodent gastrointestinal tract. Materials a...

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Published in:	Problemy osobo opasnyh infekcij no. 2; pp. 146 - 152
Main Authors:	Popova, A. Yu, Ploskireva, A. A., Kulikova, N. G., Bityumina, L. A., Chemedenko, A. F., Safandeev, V. V., Poroshin, M. A., Evdokimov, N. D., Vostrikova, M. V., Vinogradova, A. I., Latipova, R. I., Bidevkina, M. V., Bogdanova, A. V., Sinitskaya, T. A., Kuz’min, S. V., Gorelov, A. V.
Format:	Journal Article
Language:	English Russian
Published:	Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 14-07-2023
Subjects:	desulfovibrio desulfuricans iron deficiency anemia micro-biocoenosis
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Summary:	The aim of the work was to study specific systemic effect of sulfate-reducing bacteria of the genus Desulfovibrio on organism systems through creating an experimental model of intragastric bacterial invasion against the background of dysbiotic conditions of rodent gastrointestinal tract. Materials and methods . The experimental study was conducted on Wistar rats. Intestinal dysbiosis was modeled with the help of antibiotics, amoxicillin and metronidazole. Typical strain Desulfovibrio desulfuricans subsp. desulfuricans VKM B-1799 T was used as an infectious agent. Results and discussion . As a result of the experiment performed, during the period of acute infection (on day 15 of the experiment), a direct correlation between the concentration of the injected D. desulfuricans VKM B-1799 T bacteria suspension and the serum iron level was observed: the higher the infectious agent dose, the lower the serum iron concentration. During the phase of remote effects (day 38 of the experiment), the inverse correlation between the concentrations of D. desulfuricans VKM B-1799 T in the intestines of rodents was revealed: when the concentration of the infecting agent increased from 10 3 CFU/g and higher, the iron level decreased from 43.5 μmol/l to 38.5 μmol/l. When comparing the level of transferrin and unsaturated iron binding capacity (UIBC), an increase in those parameters was observed in all groups of rats both during the period of acute infection and the period of long-term effects, and the percentage of transferrin iron saturation decreased, indicating the occurrence of iron deficiency against the background of exposure to the infectious agent. Intestinal dysbiosis promotes colonization by opportunistic flora, including Desulfovibrio sp. Changes in the iron metabolism markers in experimental animals against the background of D. desulfuricans VKM B-1799 T infestation above 10 3 CFU/g and the ability of this microorganism to bind iron into an unavailable form suggest that the bacteria Desulfovibrio sp. are one of the key etiological factors of iron deficiency anemia.
ISSN:	0370-1069 2658-719X
DOI:	10.21055/0370-1069-2023-2-146-152