Comparison of the precursor, amino acid oxidation, and end-product methods for the evaluation of protein turnover in senior dogs

Stable isotope methods have been used to study protein metabolism in humans; however, there application in dogs has not been frequently explored. The present study compared the methods of precursor (13C-Leucine), end-products (15N-Glycine), and amino acid oxidation (13C-Phenylalanine) to determine t...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 19; no. 6; p. e0305073
Main Authors:	Pacheco, Letícia G, Goloni, Camila, Di Santo, Ludmilla G, Scarpim, Lucas B, Eugênio, Débora A, de Castro, Ariel, Costa, Vladimir E, Carciofi, Aulus C
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 20-06-2024 Public Library of Science (PLoS)
Subjects:	Amino acids Amino Acids - blood Amino Acids - metabolism Animals Biology and Life Sciences Blood plasma Breakdowns Carbon dioxide Carbon Isotopes Diet Dogs Expired air Female Food Glycine Glycine - blood Glycine - metabolism Glycine - urine Isotopes Laboratories Leucine Leucine - blood Leucine - metabolism Male Meals Medicine and Health Sciences Metabolism Methods Nitrogen Isotopes Nutrition research Oxidation Oxidation-Reduction Oxidation-reduction reaction Phenylalanine Phenylalanine - blood Phenylalanine - metabolism Physical Sciences Physiological aspects Physiology Precursors Protein biosynthesis Protein metabolism Protein synthesis Protein turnover Proteins Proteins - analysis Proteins - metabolism Research and Analysis Methods Respirometry Stable isotopes Statistical analysis Turnover rate Urine Variance analysis Brazil Massachusetts Nevada
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Stable isotope methods have been used to study protein metabolism in humans; however, there application in dogs has not been frequently explored. The present study compared the methods of precursor (13C-Leucine), end-products (15N-Glycine), and amino acid oxidation (13C-Phenylalanine) to determine the whole-body protein turnover rate in senior dogs. Six dogs (12.7 ± 2.6 years age, 13.6 ± 0.6 kg bodyweight) received a dry food diet for maintenance and were subjected to all the above-mentioned methods in succession. To establish 13C and 15N kinetics, according to different methodologies blood plasma, urine, and expired air were collected using a specifically designed mask. The volume of CO2 was determined using respirometry. The study included four methods viz. 13C-Leucine, 13C-Phenylalanine evaluated with expired air, 13C-Phenylalanine evaluated with urine, and 15N-Glycine, with six dogs (repetitions) per method. Data was subjected to variance analysis and means were compared using the Tukey test (P<0.05). In addition, the agreement between the methods was evaluated using Pearson correlation and Bland-Altman statistics. Protein synthesis (3.39 ± 0.33 g.kg-0,75. d-1), breakdown (3.26 ± 0.18 g.kg-0.75.d-1), and flux estimations were similar among the four methods of study (P>0.05). However, only 13C-Leucine and 13C-Phenylalanine (expired air) presented an elevated Pearson correlation and concordance. This suggested that caution should be applied while comparing the results with the other methodologies.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0305073