Search Results - "Esvelt, Kevin M."

Conservation demands safe gene drive by Esvelt, Kevin M, Gemmell, Neil J

“…Interest in developing gene drive systems to control invasive species is growing, with New Zealand reportedly considering the nascent technology as a way to…”
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Genome‐scale engineering for systems and synthetic biology by Esvelt, Kevin M, Wang, Harris H

“…Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene…”
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A system for the continuous directed evolution of biomolecules by Esvelt, Kevin M., Carlson, Jacob C., Liu, David R.

“…Speedy route to new biomolecules Many biomolecules with useful properties have been generated by laboratory molecular evolution experiments, but the processes…”
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RNA-Guided Human Genome Engineering via Cas9 by Mali, Prashant, Yang, Luhan, Esvelt, Kevin M., Aach, John, Guell, Marc, DiCarlo, James E., Norville, Julie E., Church, George M.

“…Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)…”
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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering by Mali, Prashant, Aach, John, Stranges, P Benjamin, Esvelt, Kevin M, Moosburner, Mark, Kosuri, Sriram, Yang, Luhan, Church, George M

“…A screen in human cells defines the targeting specificities of sgRNA:Cas9 and TAL-based transcriptional activators. Prokaryotic type II CRISPR-Cas systems can…”
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Concerning RNA-guided gene drives for the alteration of wild populations by Esvelt, Kevin M, Smidler, Andrea L, Catteruccia, Flaminia, Church, George M

“…Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical…”
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Bidirectional contact tracing could dramatically improve COVID-19 control by Bradshaw, William J., Alley, Ethan C., Huggins, Jonathan H., Lloyd, Alun L., Esvelt, Kevin M.

“…Contact tracing is critical to controlling COVID-19, but most protocols only “forward-trace” to notify people who were recently exposed. Using a stochastic…”
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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing by Esvelt, Kevin M, Mali, Prashant, Braff, Jonathan L, Moosburner, Mark, Yaung, Stephanie J, Church, George M

“…A set of Cas9 endonucleases orthogonal to the Streptococcus pyogenes enzyme is identified. This will enable simultaneous addressing of multiple RNA-guided…”
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Daisy-chain gene drives for the alteration of local populations by Noble, Charleston, Min, John, Olejarz, Jason, Buchthal, Joanna, Chavez, Alejandro, Smidler, Andrea L., DeBenedictis, Erika A., Church, George M., Nowak, Martin A., Esvelt, Kevin M.

“…If they are able to spread in wild populations, CRISPR-based gene-drive elements would provide new ways to address ecological problems by altering the traits…”
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Inoculating science against potential pandemics and information hazards by Esvelt, Kevin M

“…The recent de novo assembly of horsepox is an instructive example of an information hazard: published methods enabling poxvirus synthesis led to media coverage…”
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Current CRISPR gene drive systems are likely to be highly invasive in wild populations by Noble, Charleston, Adlam, Ben, Church, George M, Esvelt, Kevin M, Nowak, Martin A

“…Recent reports have suggested that self-propagating CRISPR-based gene drive systems are unlikely to efficiently invade wild populations due to drive-resistant…”
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Enabling high‐throughput biology with flexible open‐source automation by Chory, Emma J, Gretton, Dana W, DeBenedictis, Erika A, Esvelt, Kevin M

“…Our understanding of complex living systems is limited by our capacity to perform experiments in high throughput. While robotic systems have automated many…”
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Mice Against Ticks: an experimental community-guided effort to prevent tick-borne disease by altering the shared environment by Buchthal, Joanna, Evans, Sam Weiss, Lunshof, Jeantine, Telford, 3rd, Sam R, Esvelt, Kevin M

“…Mice Against Ticks is a community-guided ecological engineering project that aims to prevent tick-borne disease by using CRISPR-based genome editing to…”
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Measuring the tolerance of the genetic code to altered codon size by DeBenedictis, Erika Alden, Söll, Dieter, Esvelt, Kevin M

“…Translation using four-base codons occurs in both natural and synthetic systems. What constraints contributed to the universal adoption of a triplet codon,…”
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Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution by Dickinson, Bryan C., Leconte, Aaron M., Allen, Benjamin, Esvelt, Kevin M., Liu, David R.

“…To what extent are evolutionary outcomes determined by a population's recent environment, and to what extent do they depend on historical contingency and…”
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CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males by Smidler, Andrea L., Marrogi, Eryney, Kauffman, Jamie, Paton, Douglas G., Westervelt, Kathleen A., Church, George M., Esvelt, Kevin M., Shaw, W. Robert, Catteruccia, Flaminia

“…Rapid spread of insecticide resistance among anopheline mosquitoes threatens malaria elimination efforts, necessitating development of alternative vector…”
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A machine learning toolkit for genetic engineering attribution to facilitate biosecurity by Alley, Ethan C., Turpin, Miles, Liu, Andrew Bo, Kulp-McDowall, Taylor, Swett, Jacob, Edison, Rey, Von Stetina, Stephen E., Church, George M., Esvelt, Kevin M.

“…The promise of biotechnology is tempered by its potential for accidental or deliberate misuse. Reliably identifying telltale signatures characteristic to…”
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Analysis of the first genetic engineering attribution challenge by Crook, Oliver M., Warmbrod, Kelsey Lane, Lipstein, Greg, Chung, Christine, Bakerlee, Christopher W., McKelvey, T. Greg, Holland, Shelly R., Swett, Jacob L., Esvelt, Kevin M., Alley, Ethan C., Bradshaw, William J.

“…The ability to identify the designer of engineered biological sequences—termed genetic engineering attribution (GEA)—would help ensure due credit for…”
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The biosecurity benefits of genetic engineering attribution by Lewis, Gregory, Jordan, Jacob L., Relman, David A., Koblentz, Gregory D., Leung, Jade, Dafoe, Allan, Nelson, Cassidy, Epstein, Gerald L., Katz, Rebecca, Montague, Michael, Alley, Ethan C., Filone, Claire Marie, Luby, Stephen, Church, George M., Millett, Piers, Esvelt, Kevin M., Cameron, Elizabeth E., Inglesby, Thomas V.

“…Biology can be misused, and the risk of this causing widespread harm increases in step with the rapid march of technological progress. A key security challenge…”
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CRISPR/Cas9-mediated phage resistance is not impeded by the DNA modifications of phage T4 by Yaung, Stephanie J, Esvelt, Kevin M, Church, George M

“…Bacteria rely on two known DNA-level defenses against their bacteriophage predators: restriction-modification and Clustered Regularly Interspaced Short…”
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