Characterization of Differentially Abundant Proteins Among Leishmania (Viannia) braziliensis Strains Isolated From Atypical or Typical Lesions

is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by have been reported in several regions of the American continent, including the Xacriabá indigenous reserve in São João das Missões/Minas Gerai...

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Published in:	Frontiers in cellular and infection microbiology Vol. 12; p. 824968
Main Authors:	Esteves, Bárbara B, Melo-Braga, Marcella N, Gorshkov, Vladimir, Verano-Braga, Thiago, Larsen, Martin R, Gontijo, Célia M F, Quaresma, Patricia F, Andrade, Helida M
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 15-02-2022
Subjects:	abundance antimony resistance atypical wounds Cellular and Infection Microbiology Leishmania braziliensis proteome TMT Leishmania braziliensis antimony resistance TMT atypical wounds abundance proteome
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Summary:	is the main etiological agent of cutaneous and mucocutaneous leishmaniasis in Latin America. Non-ulcerated atypical tegumentary leishmaniasis cases caused by have been reported in several regions of the American continent, including the Xacriabá indigenous reserve in São João das Missões/Minas Gerais, Brazil. Parasites isolated from these atypical clinical lesions are resistant to antimony-based therapeutics. In the present study, proteins displaying differential abundance in two strains of isolated from patients with atypical lesions compared with four strains isolated from patients with typical lesions were identified using a quantitative proteomics approach based on tandem mass tag labeling (TMT) and mass spectrometry. A total of 532 ( <0.05) differentially abundant proteins were identified (298 upregulated and 234 downregulated) in strains from atypical lesions compared to strains from typical lesions. Prominent positively regulated proteins in atypical strains included those that may confer greater survival inside macrophages, proteins related to antimony resistance, and proteins associated with higher peroxidase activity. Additionally, we identified proteins showing potential as new drug and vaccine targets. Our findings contribute to the characterization of these intriguing strains and provide a novel perspective on Atypical Cutaneous Leishmaniasis (ACL) cases that have been associated with therapeutic failures.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Parasite and Host, a section of the journal Frontiers in Cellular and Infection Microbiology Reviewed by: Patricia Cuervo, Oswaldo Cruz Foundation (Fiocruz), Brazil; Celio Geraldo Freire-de-Lima, Federal University of Rio de Janeiro, Brazil Edited by: Juliana Perrone Bezerra De Menezes, Gonçalo Moniz Institute (IGM), Brazil
ISSN:	2235-2988 2235-2988
DOI:	10.3389/fcimb.2022.824968