Impact of after-treatment devices and biofuels on diesel exhausts genotoxicity in A549 cells exposed at air-liquid interface

Using an air-liquid interface (ALI) device in dynamic conditions, we evaluated the efficiency of fuel after-treatment strategies (diesel oxidation catalysis, DOC, and diesel particulate filter, DPF, devices) and the impact of 7% and 30% rapeseed methyl esters (RME) blending on oxidative stress and g...

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Bibliographic Details
Published in:	Toxicology in vitro Vol. 45; no. Pt 3; pp. 426 - 433
Main Authors:	Barraud, C., Corbière, C., Pottier, I., Estace, E., Blanchard, K., Logie, C., Lagadu, S., Kéravec, V., Pottier, D., Dionnet, F., Morin, J.P., Préterre, D., André, V., Monteil, C., Sichel, F.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-12-2017 Elsevier Science Ltd Elsevier
Subjects:	A549 Cells Adducts After-treatment devices Air Pollutants - analysis Air Pollutants - toxicity Air-liquid interface Biodiesel fuels Biofuels Cancer Catalase Catalase - metabolism Catalysis Cytotoxicity Deoxyribonucleic acid Diesel Diesel exhausts Diesel fuels DNA DNA Damage Esters Exhausts Fluid filters Genotoxicity Glutathione Glutathione - metabolism Health risk assessment Health risks Humans Life Sciences Lungs Mutagenicity Tests Mutagens - toxicity Oxidation Oxidative stress Oxidative Stress - drug effects Rapeseed Reactive oxygen species Reactive Oxygen Species - metabolism Risk assessment Superoxide dismutase Superoxide Dismutase - metabolism Toxicity Toxicity Tests - instrumentation Toxicity Tests - methods Vehicle Emissions - analysis Vehicle Emissions - toxicity Oxidative stress Air-liquid interface Diesel exhausts After-treatment devices Genotoxicity
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Summary:	Using an air-liquid interface (ALI) device in dynamic conditions, we evaluated the efficiency of fuel after-treatment strategies (diesel oxidation catalysis, DOC, and diesel particulate filter, DPF, devices) and the impact of 7% and 30% rapeseed methyl esters (RME) blending on oxidative stress and genotoxicity induced in A549 lung cells after 3h exposure to whole Diesel exhausts. Oxidative stress was studied using assays of ROS production, glutathione level, catalase and superoxide-dismutase (SOD) activities. No oxidative stress and no clear differences on cytotoxicity patterns between biodiesel and standard Diesel exhausts were found. A weak but significant genotoxicity (8-oxodGuo adducts) and, for standard Diesel only, a DNA damage response (DDR) as evidenced by ƔH2AX foci, remained after DOC+DPF flowing. All together, these data could contribute to the improvement of the after treatment strategies and to health risk assessment of current diesel exhausts. [Display omitted] •Effects of fuels whole exhaust were evaluated with ALI exposure of lung cells.•Biodiesels were not more pro-oxidant than standard fuel using this protocol.•A weak but significant genotoxicity was found with Diesel and Biodiesel with 7%RME.•Genotoxicity remained after post-treatment devices (DOC+DPF).
ISSN:	0887-2333 1879-3177
DOI:	10.1016/j.tiv.2017.04.025