Polymorphisms of the FTO and MTHFR genes and vascular, inflammatory and metabolic marker levels in postmenopausal women

Polymorphisms of the FTO and MTHFR genes and vascular, inflammatory and metabolic marker levels in postmenopausal women

Objective To determine the prevalence of three single nucleotide polymorphisms (SNPs) in postmenopausal women with and without the metabolic syndrome (METS) and to explore levels of circulating biomarkers of inflammation, vascular and metabolic dysfunction according to SNP genotypes. Methods DNA was...

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Bibliographic Details
Published in:Journal of endocrinological investigation Vol. 39; no. 8; pp. 885 - 890
Main Authors: Chedraui, P., Pérez-López, F. R., Escobar, G. S., Espinoza-Caicedo, J. A., Montt-Guevara, M., Genazzani, A. R., Simoncini, T.
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-08-2016
Subjects:
Adult
Aged
Alpha-Ketoglutarate-Dependent Dioxygenase FTO - genetics
Biomarkers - blood
Case-Control Studies
Endocrinology
Female
Humans
Inflammation - blood
Inflammation - genetics
Medicine
Medicine & Public Health
Metabolic Diseases
Metabolic Syndrome - complications
Metabolic Syndrome - physiopathology
Methylenetetrahydrofolate Reductase (NADPH2) - genetics
Middle Aged
Obesity - complications
Original Article
Polymerase Chain Reaction
Polymorphism, Single Nucleotide - genetics
Postmenopause
Vascular Diseases - blood
Vascular Diseases - genetics
FTO
sCD40L
Visfatin
Postmenopause
MTHFR
Metabolic syndrome
Polymorphisms
Genotypes
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Summary:Objective To determine the prevalence of three single nucleotide polymorphisms (SNPs) in postmenopausal women with and without the metabolic syndrome (METS) and to explore levels of circulating biomarkers of inflammation, vascular and metabolic dysfunction according to SNP genotypes. Methods DNA was extracted from the whole blood of 192 natural postmenopausal women (40 to 65 years) screened for the METS and tested for three gene SNPs related to obesity: the fat mass obesity (FTO: rs9939609) and the methylenetetrahydrofolate reductase (MTHFR: C677T and A1298C). Blood levels of angiopoietin, IL-8, sFASL, IL-6, TNF-α, sCD40L, PAI-1, u-PA, leptin, adiponectin, resistin, ghrelin, visfatin, adipsin and insulin were measured in a subgroup, with and without the METS, using multiplex technology ( n  = 100) and compared according to SNP genotypes. Results Genotype frequency of the three studied SNPs did not differ in relation to the presence of the METS. However, genotypes CT+TT (C677T) and AT (rs9939609) were more prevalent in women with high triglyceride levels. Pooled sub-analysis ( n  = 100) found that median sCD40L and visfatin levels were higher in women with genotypes AT+TT (rs9939609) as compared to AA (1178 vs. 937.0 pg/mL and 0.93 vs. 0.43 ng/mL, respectively, p  < 0.05). Conclusion Two SNP genotypes related to obesity were more prevalent in women with abnormal triglyceride levels and two vascular and inflammatory serum markers were higher in relation to the rs9939609 SNP.
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ISSN:1720-8386
1720-8386
DOI:10.1007/s40618-016-0443-7