Random amplified polymorphic DNA (RAPD) analysis of genotypic identities in Eucalyptus clones

Vegetative micropropagation is usually applied in Eucalyptus in order to obtain clones for improvement on plant propagation for commercial purposes. One problem of this technique is somaclonal variation, which serves as a source of undesirable genetic variation, in a propagation of previously select...

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Bibliographic Details
Published in:	Silvae genetica Vol. 49; no. 6; pp. 239 - 243
Main Authors:	Delaia, M.L, Gomes, E.A, Esbrisse, E.J, Dearaujo, E.F
Format:	Journal Article
Language:	English
Published:	Frankfurt am Main Sauerländer 2000
Subjects:	ADN Agronomy. Soil science and plant productions Artificial regeneration. Forest nurseries. Planting Biological and medical sciences Biotechnology Classical and quantitative genetics. Population genetics. Molecular genetics CLONE CLONES Cultivars: description, identification, tests, catalogs DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE DNA EMBRYONIC DEVELOPMENT EUCALYPTUS Forestry Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetic resources, diversity Genetics and breeding of economic plants GENOTIPOS GENOTYPE GENOTYPES Molecular genetics Plant material Varietal selection. Specialized plant breeding, plant breeding aims Vegetative propagation. Micropropagation South America Brazil America Biotechnology Genetic marker PROPAGATION MATERIALS Genotype Micropropagation Experimental study Somaclonal variation Random amplified polymorphic DNA marker Eucalyptus Dicotyledones DNA Genetic improvement Angiospermae Myrtaceae Genetic identification Spermatophyta Molecular biology Clone Method study
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Summary:	Vegetative micropropagation is usually applied in Eucalyptus in order to obtain clones for improvement on plant propagation for commercial purposes. One problem of this technique is somaclonal variation, which serves as a source of undesirable genetic variation, in a propagation of previously selected clones. To analyze the genotypes, Eucalyptus clones hybrids obtained by vegetative micropropagation were evaluated by RAPD markers. Fifteen arbitrary 10-mer primers were successfully used to amplify DNA of four clones obtained in different subcultures from callus to adult plants. During the analysis of clone ''A'' polymorphism was observed in the pattern of fragments of amplified DNA among subcultures, producing 39 polymorphic and 23 monomorphic bands. The genetic distance varied from 0 to 37% within this clone. For clones ''B'', ''C'' and ''D'' no polymorphism was observed in all plants in different ages. These results suggest the existence of sample exchange or somaclonal variation in clone ''A'' and showed that RAPD markers are an efficient tool for the early analysis of genotypes in Eucalyptus clones.
Bibliography:	F30 2001T30020 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0037-5349 2509-8934