Search Results - "Esbjörner, Elin K"

Delivery of Oligonucleotide Therapeutics: Chemical Modifications, Lipid Nanoparticles, and Extracellular Vesicles by Bost, Jeremy P, Barriga, Hanna, Holme, Margaret N, Gallud, Audrey, Maugeri, Marco, Gupta, Dhanu, Lehto, Taavi, Valadi, Hadi, Esbjörner, Elin K, Stevens, Molly M, El-Andaloussi, Samir

“…Oligonucleotides (ONs) comprise a rapidly growing class of therapeutics. In recent years, the list of FDA-approved ON therapies has rapidly expanded. ONs are…”
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Graphene oxide sheets and quantum dots inhibit α-synuclein amyloid formation by different mechanisms by Ghaeidamini, Marziyeh, Bernson, David, Sasanian, Nima, Kumar, Ranjeet, Esbjörner, Elin K

“…Aggregation and amyloid formation of the 140-residue presynaptic and intrinsically disordered protein α-synuclein (α-syn) is a pathological hallmark of…”
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Interaction Kinetics of Individual mRNA-Containing Lipid Nanoparticles with an Endosomal Membrane Mimic: Dependence on pH, Protein Corona Formation, and Lipoprotein Depletion by Aliakbarinodehi, Nima, Gallud, Audrey, Mapar, Mokhtar, Wesén, Emelie, Heydari, Sahar, Jing, Yujia, Emilsson, Gustav, Liu, Kai, Sabirsh, Alan, Zhdanov, Vladimir P., Lindfors, Lennart, Esbjörner, Elin K., Höök, Fredrik

“…Lipid nanoparticles (LNPs) have emerged as potent carriers for mRNA delivery, but several challenges remain before this approach can offer broad clinical…”
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Lipid membranes catalyse the fibril formation of the amyloid-β (1–42) peptide through lipid-fibril interactions that reinforce secondary pathways by Lindberg, David J., Wesén, Emelie, Björkeroth, Johan, Rocha, Sandra, Esbjörner, Elin K.

“…Alzheimer's disease is associated with the aggregation of amyloid-β (Aβ) peptides into oligomers and fibrils. We have explored how model lipid membranes…”
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Single-shot self-supervised object detection in microscopy by Midtvedt, Benjamin, Pineda, Jesús, Skärberg, Fredrik, Olsén, Erik, Bachimanchi, Harshith, Wesén, Emelie, Esbjörner, Elin K., Selander, Erik, Höök, Fredrik, Midtvedt, Daniel, Volpe, Giovanni

“…Object detection is a fundamental task in digital microscopy, where machine learning has made great strides in overcoming the limitations of classical…”
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Binding of Thioflavin‑T to Amyloid Fibrils Leads to Fluorescence Self-Quenching and Fibril Compaction by Lindberg, David J, Wenger, Anna, Sundin, Elin, Wesén, Emelie, Westerlund, Fredrik, Esbjörner, Elin K

“…Thioflavin-T binds to and detects amyloid fibrils via fluorescence enhancement. Using a combination of linear dichroism and fluorescence spectroscopies, we…”
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Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery by Baladi, Tom, Nilsson, Jesper R, Gallud, Audrey, Celauro, Emanuele, Gasse, Cécile, Levi-Acobas, Fabienne, Sarac, Ivo, Hollenstein, Marcel R, Dahlén, Anders, Esbjörner, Elin K, Wilhelmsson, L. Marcus

“…Methods for tracking RNA inside living cells without perturbing their natural interactions and functions are critical within biology and, in particular, to…”
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Effects of Tryptophan Content and Backbone Spacing on the Uptake Efficiency of Cell-Penetrating Peptides by Rydberg, Hanna A, Matson, Maria, Åmand, Helene L, Esbjörner, Elin K, Nordén, Bengt

“…Cell-penetrating peptides (CPPs) are able to traverse cellular membranes and deliver macromolecular cargo. Uptake occurs through both endocytotic and…”
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The inhibition of fibril formation of lysozyme by sucrose and trehalose by Ahlgren, Kajsa, Havemeister, Fritjof, Andersson, Julia, Esbjörner, Elin K, Swenson, Jan

“…The two disaccharides, trehalose and sucrose, have been compared in many studies due to their structural similarity. Both possess the ability to stabilise and…”
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Endocytic uptake of monomeric amyloid-β peptides is clathrin- and dynamin-independent and results in selective accumulation of Aβ(1–42) compared to Aβ(1–40) by Wesén, Emelie, Jeffries, Gavin D. M., Matson Dzebo, Maria, Esbjörner, Elin K.

“…Intraneuronal accumulation of amyloid-β (Aβ) peptides represent an early pathological feature in Alzheimer’s disease. We have therefore utilized flow cytometry…”
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Steady-state and time-resolved Thioflavin-T fluorescence can report on morphological differences in amyloid fibrils formed by Aβ(1-40) and Aβ(1-42) by Lindberg, David J., Wranne, Moa S., Gilbert Gatty, Mélina, Westerlund, Fredrik, Esbjörner, Elin K.

“…Thioflavin-T (ThT) is one of the most commonly used dyes for amyloid detection, but the origin of its fluorescence enhancement is not fully understood. Herein…”
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The Liver and Kidneys mediate clearance of cardiac troponin in the rat by Muslimovic, Aida, Fridén, Vincent, Tenstad, Olav, Starnberg, Karin, Nyström, Susanne, Wesén, Emelie, Esbjörner, Elin K., Granholm, Kristoffer, Lindahl, Bertil, Hammarsten, Ola

“…Cardiac-specific troponins (cTn), troponin T (cTnT) and troponin I (cTnI) are diagnostic biomarkers when myocardial infarction is suspected. Despite its…”
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Cell surface binding and uptake of arginine- and lysine-rich penetratin peptides in absence and presence of proteoglycans by Åmand, Helene L., Rydberg, Hanna A., Fornander, Louise H., Lincoln, Per, Nordén, Bengt, Esbjörner, Elin K.

“…Cell surface proteoglycans (PGs) appear to promote uptake of arginine-rich cell-penetrating peptides (CPPs), but their exact functions are unclear. To address…”
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Extracellular Vesicles Slow Down Aβ(1–42) Aggregation by Interfering with the Amyloid Fibril Elongation Step by Halipi, Vesa, Sasanian, Nima, Feng, Julia, Hu, Jing, Lubart, Quentin, Bernson, David, van Leeuwen, Daniel, Ahmadpour, Doryaneh, Sparr, Emma, Esbjörner, Elin K.

“…Formation of amyloid-β (Aβ) fibrils is a central pathogenic feature of Alzheimer’s disease. Cell-secreted extracellular vesicles (EVs) have been suggested as…”
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Correlation between Cellular Uptake and Cytotoxicity of Fragmented α‑Synuclein Amyloid Fibrils Suggests Intracellular Basis for Toxicity by Zhang, Xiaolu, Wesén, Emelie, Kumar, Ranjeet, Bernson, David, Gallud, Audrey, Paul, Alexandra, Wittung-Stafshede, Pernilla, Esbjörner, Elin K

“…Aggregation and intracellular deposition of the protein α-synuclein is an underlying characteristic of Parkinson’s disease. α-Synuclein assemblies also undergo…”
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Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles by Špačková, Barbora, Klein Moberg, Henrik, Fritzsche, Joachim, Tenghamn, Johan, Sjösten, Gustaf, Šípová-Jungová, Hana, Albinsson, David, Lubart, Quentin, van Leeuwen, Daniel, Westerlund, Fredrik, Midtvedt, Daniel, Esbjörner, Elin K., Käll, Mikael, Volpe, Giovanni, Langhammer, Christoph

“…Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is…”
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Metabolic RNA labeling in non-engineered cells following spontaneous uptake of fluorescent nucleoside phosphate analogues by Pfeiffer, Pauline, Nilsson, Jesper R, Gallud, Audrey, Baladi, Tom, Le, Hoang-Ngoan, Bood, Mattias, Lemurell, Malin, Dahlén, Anders, Grøtli, Morten, Esbjörner, Elin K, Wilhelmsson, L Marcus

“…RNA and its building blocks play central roles in biology and have become increasingly important as therapeutic agents and targets. Hence, probing and…”
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A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery by Munson, Michael J., O’Driscoll, Gwen, Silva, Andreia M., Lázaro-Ibáñez, Elisa, Gallud, Audrey, Wilson, John T., Collén, Anna, Esbjörner, Elin K., Sabirsh, Alan

“…RNA-based therapies have great potential to treat many undruggable human diseases. However, their efficacy, in particular for mRNA, remains hampered by poor…”
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Independent Size and Fluorescence Emission Determination of Individual Biological Nanoparticles Reveals that Lipophilic Dye Incorporation Does Not Scale with Particle Size by Jõemetsa, Silver, Joyce, Paul, Lubart, Quentin, Mapar, Mokhtar, Celauro, Emanuele, Agnarsson, Björn, Block, Stephan, Bally, Marta, Esbjörner, Elin K, Jeffries, Gavin D.M, Höök, Fredrik

“…Advancements in nanoparticle characterization techniques are critical for improving the understanding of how biological nanoparticles (BNPs) contribute to…”
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Fluorescent base analogues in gapmers enable stealth labeling of antisense oligonucleotide therapeutics by Nilsson, Jesper R., Baladi, Tom, Gallud, Audrey, Baždarević, Dženita, Lemurell, Malin, Esbjörner, Elin K., Wilhelmsson, L. Marcus, Dahlén, Anders

“…To expand the antisense oligonucleotide (ASO) fluorescence labeling toolbox beyond covalent conjugation of external dyes ( e.g. ATTO-, Alexa Fluor-, or cyanine…”
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