The cagA status of Helicobacter pylori isolates from dyspeptic children in Turkey

There are inconsistent reports regarding cytotoxin-associated gene A ( cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA + strains and to evaluate its correlation with clinic and endoscopic...

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Bibliographic Details
Published in:	FEMS immunology and medical microbiology Vol. 36; no. 3; pp. 147 - 149
Main Authors:	Saltık, İnci Nur, Demir, Hülya, Engin, Doruk, Ertunç, Özge Darka, Akyön, Yakut, Koçak, Nurten
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Oxford, UK Elsevier B.V 25-05-2003 Blackwell Publishing Ltd Blackwell
Subjects:	Adolescent Antigens, Bacterial - genetics Antral nodularity Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacterial Proteins - genetics Bacteriology Biological and medical sciences Biopsy cagA Child Children DNA, Bacterial - analysis Dyspepsia - epidemiology Dyspepsia - microbiology Dyspepsia - physiopathology Epidemiology Female Fundamental and applied biological sciences. Psychology Helicobacter Infections - epidemiology Helicobacter Infections - microbiology Helicobacter Infections - physiopathology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Helicobacter pylori - pathogenicity Human bacterial diseases Humans Infectious diseases Male Medical sciences Microbiology Polymerase Chain Reaction Prevalence Pyloric Antrum - pathology Severity of Illness Index Turkey - epidemiology Asia Turkey Antral nodularity Children Helicobacter pylori cagA Human Prevalence Spirillales Dyspepsia Spirillaceae Genotype Pathogenicity island Infection Bacteriosis Digestive diseases Bacteria Veterinary Child Clinical isolate
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Summary:	There are inconsistent reports regarding cytotoxin-associated gene A ( cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA + strains and to evaluate its correlation with clinic and endoscopic findings. We examined 45 H. pylori strains that were grown on brain–heart infusion agar supplemented with 7% horse blood. Following 72 h of incubation colonies were harvested and bacterial DNA was extracted. Polymerase chain reaction primers F1 and B1 were used to amplify a 348-bp internal fragment of cagA. The prevalence of cagA in Turkish pediatric patients was 55.6%. No association was found between cagA status and the severity of gastro-duodenal lesions.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0928-8244 1574-695X
DOI:	10.1016/S0928-8244(03)00024-5