Biocatalytic assembly of chemically modified oligonucleotides

Biocatalytic assembly of chemically modified oligonucleotides

[Display omitted] With renewed interest in therapeutic oligonucleotides, and at the same time the pharmaceutical industry set itself ambitious sustainability goals, there is a strong interest to investigate next-generation synthesis methods for these highly modified oligonucleotides. We herein repor...

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Bibliographic Details
Published in:Tetrahedron letters Vol. 93; p. 153696
Main Authors: Mann, Greg, Stanger, Frédéric V., Peschke, Theo, Schroer, Kirsten, Trung, Nhat Quang Nguyen, Burrer, Nathalie, Grassinger, Franziska, Piccioni, Lorenzo, Erdogan, Oemer, Heimburger, Jeremy, Koehl, Hervé, Ertle, Heike, Denay, Regis, Dreier, Philippe, Schlama, Thierry
Format: Journal Article
Language:English
Published: Elsevier Ltd 16-03-2022
Subjects:
Biocatalysis
Ligation
Oligonucleotides
Sustainable
Biocatalysis
Ligation
Sustainable
Oligonucleotides
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Summary:[Display omitted] With renewed interest in therapeutic oligonucleotides, and at the same time the pharmaceutical industry set itself ambitious sustainability goals, there is a strong interest to investigate next-generation synthesis methods for these highly modified oligonucleotides. We herein report an RNA ligase catalyzed assembly of multiple, short oligonucleotides in aqueous solution to generate an siRNA product. A conversion of the non-native substrates to the desired product of 40 – 80 % was achieved.
ISSN:0040-4039
1873-3581
DOI:10.1016/j.tetlet.2022.153696