Human papillomavirus, Epstein–Barr virus, and Candida albicans co‐infection in oral leukoplakia with different degrees of dysplasia

Human papillomavirus, Epstein–Barr virus, and Candida albicans co‐infection in oral leukoplakia with different degrees of dysplasia

Objectives To identify human papillomavirus (HPV), Epstein–Barr virus (EBV), and Candida albicans in oral leukoplakia with different degrees of dysplasia. Materials and methods An observational, cross‐sectional, descriptive study was performed using 30 formalin‐fixed and paraffin‐embedded tissues fr...

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Bibliographic Details
Published in:Clinical and experimental dental research Vol. 7; no. 5; pp. 914 - 923
Main Authors: Erira, Alveiro T, Navarro, Andrea Fernanda Romo, Robayo, Dabeiba Adriana García
Format: Journal Article
Language:English
Published: United States John Wiley & Sons, Inc 01-10-2021
John Wiley and Sons Inc
Wiley
Subjects:
Alphapapillomavirus
Biopsy
Candida albicans
Coinfection
Cross-Sectional Studies
Documentation
dysplasia
Epstein-Barr virus
Epstein-Barr Virus Infections - complications
Epstein-Barr Virus Infections - epidemiology
Experiments
Female
Herpesvirus 4, Human - genetics
Human papillomavirus
Humans
Infections
leukoplakia
Leukoplakia, Oral
Middle Aged
Oral cancer
Original
Papillomaviridae
Papillomavirus Infections - complications
Thermal cycling
Candida albicans
dysplasia
Human papillomavirus
Epstein-Barr virus
leukoplakia
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Summary:Objectives To identify human papillomavirus (HPV), Epstein–Barr virus (EBV), and Candida albicans in oral leukoplakia with different degrees of dysplasia. Materials and methods An observational, cross‐sectional, descriptive study was performed using 30 formalin‐fixed and paraffin‐embedded tissues from patients with clinical suspicion of leukoplakia and confirmed diagnosis of oral dysplasia. Histological analyses were performed by two pathologists (interobserver) and dysplasias were classified as mild, moderate, or severe. Conventional PCR was used to detect HPV and EBV viruses and C. albicans. To determine the association between each microorganism with different degrees of dysplasia a Chi‐square test was employed. Results The tongue was the most common site for leukoplakias (71.4%) in females with a mean age of 50 years (ranging between 30 to 50 years old; 57.1%). EBV was the most frequently detected (73.3%), followed by HPV (43.3%), mainly of type 16 (40%), and C. albicans (23.3%). Significant differences were observed between degrees of dysplasia and HPV presence (p = 0.005). In lesions positive for HPV, EBV, and C. albicans the most frequent histological changes were hyperkeratosis, irregular interpapillary ridges, and loss of basal stratum cell polarity. Conclusion Co‐infection with human papillomavirus, Epstein Barr virus, and Candida albicans in oral leukoplakia could be associated with dysplastic changes.
Bibliography:Funding information
Universidad Cooperativa de Colombia ‐ Bogotá Colombia, Grant/Award Number: INV1683
Partial results of this work were presented as an oral presentation at the National Dental Research 2017 conference (ACFO‐2017) in Cartagena – Colombia.
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Funding information Universidad Cooperativa de Colombia ‐ Bogotá Colombia, Grant/Award Number: INV1683
ISSN:2057-4347
2057-4347
DOI:10.1002/cre2.435