Search Results - "Eric Plum, G"

Dynamic DNA Energy Landscapes and Substrate Complexity in Triplet Repeat Expansion and DNA Repair by Völker, Jens, Plum, G Eric, Gindikin, Vera, Breslauer, Kenneth J

“…DNA repeat domains implicated in DNA expansion diseases exhibit complex conformational and energy landscapes that impact biological outcomes. These landscapes…”
Get full text

Impact of bulge loop size on DNA triplet repeat domains: Implications for DNA repair and expansion by Völker, Jens, Plum, G. Eric, Gindikin, Vera, Klump, Horst H., Breslauer, Kenneth J.

“…ABSTRACT Repetitive DNA sequences exhibit complex structural and energy landscapes, populated by metastable, noncanonical states, that favor expansion and…”
Get full text

Energy Crosstalk between DNA Lesions: Implications for Allosteric Coupling of DNA Repair and Triplet Repeat Expansion Pathways by Völker, Jens, Plum, G. Eric, Klump, Horst H, Breslauer, Kenneth J

“…Energy coupling between distal DNA domains may have profound regulatory consequences for biological processes, allowing for allosteric control of nucleic acid…”
Get full text

A More Unified Picture for the Thermodynamics of Nucleic Acid Duplex Melting: A Characterization by Calorimetric and Volumetric Techniques by Chalikian, Tigran V., Völker, Jens, Plum, G. Eric, Breslauer, Kenneth J.

“…We use a combination of calorimetric and volumetric techniques to detect and to characterize the thermodynamic changes that accompany helix-to-coil transitions…”
Get full text

Energetic coupling between clustered lesions modulated by intervening triplet repeat bulge loops: Allosteric implications for DNA repair and triplet repeat expansion by Völker, Jens, Plum, G. Eric, Klump, Horst H., Breslauer, Kenneth J.

“…Clusters of closely spaced oxidative DNA lesions present challenges to the cellular repair machinery. When located in opposing strands, base excision repair…”
Get full text

Heat Capacity Changes (ΔC p ) for Interconversions between Differentially-Ordered DNA States within Physiological Temperature Domains: Implications for Biological Regulatory Switches by Völker, Jens, Plum, G. Eric, Breslauer, Kenneth J

“…Knowledge of differences in heat capacity changes (ΔC p ) between biopolymer states provides essential information about the temperature dependence of the…”
Get full text

The Thermodynamics of Template-Directed DNA Synthesis: Base Insertion and Extension Enthalpies by Conceição A. S. A. Minetti, Remeta, David P., Miller, Holly, Gelfand, Craig A., Plum, G. Eric, Grollman, Arthur P., Breslauer, Kenneth J.

“…We used stopped-flow calorimetry to measure the overall enthalpy change associated with template-directed nucleotide insertion and DNA extension. Specifically,…”
Get full text

Energetics of Lesion Recognition by a DNA Repair Protein: Thermodynamic Characterization of Formamidopyrimidine-glycosylase (Fpg) Interactions with Damaged DNA Duplexes by Minetti, Conceição A.S.A, Remeta, David P, Zharkov, Dmitry O, Eric Plum, G, Johnson, Francis, Grollman, Arthur P, Breslauer, Kenneth J

“…As part of an overall effort to map the energetic landscape of the base excision repair pathway, we report the first thermodynamic characterization of repair…”
Get full text

Thermodynamics of an Intramolecular DNA Triple Helix: A Calorimetric and Spectroscopic Study of the pH and Salt Dependence of Thermally Induced Structural Transitions by Plum, Eric G., Breslauer, Kenneth J.

“…We have characterized thermodynamically the melting transitions of a DNA 31-mer oligonucleotide (5′-GAAGAGGTTTTTCCTCTTCTTTTTCTTCTCC-3′) which is designed to…”
Get full text

Energy Landscapes of Dynamic Ensembles of Rolling Triplet Repeat Bulge Loops: Implications for DNA Expansion Associated with Disease States by Völker, Jens, Gindikin, Vera, Klump, Horst H., Plum, G. Eric, Breslauer, Kenneth J.

“…DNA repeat domains can form ensembles of canonical and noncanonical states, including stable and metastable DNA secondary structures. Such sequence-induced…”
Get full text

DNA Repair and DNA Triplet Repeat Expansion: The Impact of Abasic Lesions on Triplet Repeat DNA Energetics by Völker, Jens, Plum, G. Eric, Klump, Horst H, Breslauer, Kenneth J

“…Enhanced levels of DNA triplet expansion are observed when base excision repair (BER) of oxidative DNA base damage (e.g., 8-oxo-dG) occurs at or near CAG…”
Get full text

A Quantitative Method for Evaluating the Stabilities of Nucleic Acids by Gelfand, Craig A., Plum, G. Eric, Mielewczyk, Slawomir, Remeta, David P., Breslauer, Kenneth J.

“…We report a general method for screening, in solution, the impact of deviations from canonical Watson-Crick composition on the thermodynamic stability of…”
Get full text

DNA meter: Energy tunable, quantitative hybridization assay by Braunlin, William, Völker, Jens, Plum, G. Eric, Breslauer, Kenneth J.

“…We describe a novel hybridization assay that employs a unique class of energy tunable, bulge loop‐containing competitor strands (C*) that hybridize to a probe…”
Get full text

Thermodynamic Consequences of an Abasic Lesion in Duplex DNA Are Strongly Dependent on Base Sequence by Gelfand, Craig A, Plum, G. Eric, Grollman, Arthur P, Johnson, Francis, Breslauer, Kenneth J

“…The abasic site in DNA may arise spontaneously, as a result of nucleotide base damage, or as an intermediate in glycosylase-mediated DNA-repair pathways. It is…”
Get full text

Influence of the Oxidatively Damaged Adduct 8-Oxodeoxyguanosine on the Conformation, Energetics, and Thermodynamic Stability of a DNA Duplex by Plum, G. Eric, Grollman, Arthur P, Johnson, Francis, Breslauer, Kenneth J

“…As part of an overall program to characterize the impact of mutagenic lesions on the physiochemical properties of DNA, we report here the results of a…”
Get full text

High resolution protein-DNA binding energy landscapes via a novel high throughput method by Plum, G. Eric, Breslauer, David N., Breslauer, Kenneth J.

Get full text

The thermodynamics of DNA structures that contain lesions of guanine tetrads by Pilch, Daniel S, Plum, G Eric, Breslauer, Kenneth J

“…It is becoming increasingly apparent that energetic as well as structural information is required to develop a complete appreciation of the critical…”
Get full text

Thermodynamic Characterization of the Stability and the Melting Behavior of a DNA Triplex: A Spectroscopic and Calorimetric Study by Plum, G. Eric, Park, Young-Whan, Singleton, Scott F., Dervan, Peter B., Breslauer, Kenneth J.

“…We report a complete thermodynamic characterization of the stability and the melting behavior of an oligomeric DNA triplex. The triplex chosen for study forms…”
Get full text

Calorimetry of proteins and nucleic acids by Plum, G Eric, Breslauer, Kenneth J

“…The availability of sensitive calorimetric instrumentation has led to a considerable increase in thermodynamic studies of proteins, nucleic acids, and their…”
Get full text

Berenil [1,3-Bis(4'-amidinophenyl)triazene] Binding to DNA Duplexes and to a RNA Duplex: Evidence for Both Intercalative and Minor Groove Binding Properties by Pilch, Daniel S, Kirolos, Michael A, Liu, Xiaoyan, Plum, G. Eric, Breslauer, Kenneth J

Get full text