3D bio-printing of levan/polycaprolactone/gelatin blends for bone tissue engineering: Characterization of the cellular behavior

[Display omitted] •3D printed scaffolds of the PCL/GT/HLh blends were successfully fabricated and characterized.•This is the first 3D bioprinting study of the levan containing formulations.•Significant cell viability was observed.•Halomonas levan increased the Hob proliferation.•The obtained 3D scaf...

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Bibliographic Details
Published in:	European polymer journal Vol. 119; pp. 426 - 437
Main Authors:	Duymaz, Busra Tugce, Erdiler, Fatma Betul, Alan, Tugba, Aydogdu, Mehmet Onur, Inan, Ahmet Talat, Ekren, Nazmi, Uzun, Muhammet, Sahin, Yesim Muge, Bulus, Erdi, Oktar, Faik Nüzhet, Selvi, Sinem Selvin, ToksoyOner, Ebru, Kilic, Osman, Bostan, Muge Sennaroglu, Eroglu, Mehmet Sayip, Gunduz, Oguzhan
Format:	Journal Article
Language:	English
Published:	Oxford Elsevier Ltd 01-10-2019 Elsevier BV
Subjects:	3-D technology 3D printing Biocompatibility Biomedical materials Cell adhesion Cellular biology Fourier transforms Gelatin Halomonas levan Infrared spectroscopy Low molecular weights Molecular weight Morphology Physical properties Polycaprolactone Scaffolds Scanning electron microscopy Surface tension Three dimensional printing Tissue engineering Tissue engineering 3D printing Halomonas levan
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Summary:	[Display omitted] •3D printed scaffolds of the PCL/GT/HLh blends were successfully fabricated and characterized.•This is the first 3D bioprinting study of the levan containing formulations.•Significant cell viability was observed.•Halomonas levan increased the Hob proliferation.•The obtained 3D scaffold had a uniform pore size with homogeneous distribution. Poly(ε-caprolactone) (PCL), gelatin (GT) and different concentrations of low molecular weight Halomonas levan (HLh) were combined and examined to develop physical networks serving as tissue scaffolds to promote cell adhesion for biocompatibility. Three-dimensional bioprinting technique (3D bioprinting) was employed during manufacturing the test samples and their comprehensive characterization was performed to investigate the physicochemical properties and biocompatibility. Physical properties of the printing materials such as viscosity, surface tension, and density were measured to determine optimal parameters for 3D bioprinting. The scanning electron microscope (SEM) was used to observe the morphological structure of scaffolds. Fourier-Transform Infrared Spectroscopy (FT-IR) and differential scanning calorimetry (DSC) were used to identify the interactions between the components. In-vitro cell culture assays using standard human osteoblast (Hob) cells showed increased biocompatibility of the printing materials with increasing HLh content. Thus, the formulations including the HLh are expected to be a good candidate for the production of 3D printed materials.
ISSN:	0014-3057 1873-1945
DOI:	10.1016/j.eurpolymj.2019.08.015