Detection and Investigation of Extracellular Vesicles in Serum and Urine Supernatant of Prostate Cancer Patients

Detection and Investigation of Extracellular Vesicles in Serum and Urine Supernatant of Prostate Cancer Patients

Prostate Cancer (PCa) is one of the most frequently identified urological cancers. PCa patients are often over-diagnosed due to still not highly specific diagnostic methods. The need for more accurate diagnostic tools to prevent overestimated diagnosis and unnecessary treatment of patients with non-...

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Bibliographic Details
Published in:Diagnostics (Basel) Vol. 11; no. 3; p. 466
Main Authors: Salvi, Samanta, Bandini, Erika, Carloni, Silvia, Casadio, Valentina, Battistelli, Michela, Salucci, Sara, Erani, Ilaria, Scarpi, Emanuela, Gunelli, Roberta, Cicchetti, Giacomo, Guescini, Michele, Bonafè, Massimiliano, Fabbri, Francesco
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 08-03-2021
MDPI
Subjects:
Antigens
Biopsy
Clinical medicine
Extracellular vesicles
Feasibility studies
MACSPlex Exosome kit
Prostate cancer
Proteins
Reagents
serum
Software
Statistical analysis
Urine
United Kingdom > UK
United States > US
Italy
prostate cancer
extracellular vesicles
serum
urine
MACSPlex Exosome kit
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Summary:Prostate Cancer (PCa) is one of the most frequently identified urological cancers. PCa patients are often over-diagnosed due to still not highly specific diagnostic methods. The need for more accurate diagnostic tools to prevent overestimated diagnosis and unnecessary treatment of patients with non-malignant conditions is clear, and new markers and methods are strongly desirable. Extracellular vesicles (EVs) hold great promises as liquid biopsy-based markers. Despite the biological and technical issues present in their detection and study, these particles can be found highly abundantly in the biofluid and encompass a wealth of macromolecules that have been reported to be related to many physiological and pathological processes, including cancer onset, metastasis spreading, and treatment resistance. The present study aims to perform a technical feasibility study to develop a new workflow for investigating EVs from several biological sources. Serum and urinary supernatant EVs of PCa, benign prostatic hyperplasia (BPH) patients, and healthy donors were isolated and investigated by a fast, easily performable, and cost-effective cytofluorimetric approach for a multiplex detection of 37 EV-antigens. We also observed significant alterations in serum and urinary supernatant EVs potentially related to BPH and PCa, suggesting a potential clinical application of this workflow.
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These authors contributed equally to this work.
ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics11030466