Phenotypic characterization and epidemiology of extended-spectrum β -lactamase-producing Enterobacteriaceae strains from urinary tract infections in Garoua, Cameroon

The emergence of extended-spectrum beta-lactamase-producing (ESBL-E) is causing increased morbidity and mortality around the world as a result of therapeutic failures. ESBL-E are priority pathogens due to their multidrug resistance (MDR). In Northern Cameroon, ESBL-producing bacteria, particularly i...

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Published in:	Frontiers in public health Vol. 11; p. 1187934
Main Authors:	Djim-Adjim-Ngana, Karyom, Mbiakop, Brunel W, Oumar, Leila A, Munshili Njifon, Hermann L, Tchinda Fossi, Cedric, Enyegue, Elisee L Embolo, Mouiche Mouliom, Mohamed M, Fodouop Chegaing, Siméon P, Deweerdt, Louis, Yanou, Nicolas Njintang, Nguinkal, Julien A
Format:	Journal Article
Language:	English
Published:	Switzerland Frontiers Media S.A 29-06-2023
Subjects:	Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use antibiotics beta-Lactamases Cameroon - epidemiology double synergy test Enterobacteriaceae ESBL Escherichia coli Humans Microbial Sensitivity Tests Public Health Retrospective Studies Urinary Tract Infections - drug therapy Urinary Tract Infections - epidemiology Urinary Tract Infections - microbiology UTI—Urinary tract infection Cameroon double synergy test multidrug resistance ESBL microbial susceptibility UTI—Urinary tract infection antibiotics Enterobacteriaceae
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Summary:	The emergence of extended-spectrum beta-lactamase-producing (ESBL-E) is causing increased morbidity and mortality around the world as a result of therapeutic failures. ESBL-E are priority pathogens due to their multidrug resistance (MDR). In Northern Cameroon, ESBL-producing bacteria, particularly in urinary tract infections (UTIs), are being increasingly isolated. This study aimed to retrospectively determine the prevalence of multi-drug resistant ESBL strains isolated from UTIs in Northern Cameroon and to evaluate the effectiveness of the ATB UR Gallery of BioMérieux in diagnosing ESBL-E in clinical settings. Standard microbiology protocols and statistical tools were utilized to identify ESBL-producing bacteria and characterize their phenotypic susceptibility and resistance profiles in the study population. Out of the 144 enterobacteria isolates successfully cultured, 59 (41%) were identified as MDR strains. The ATB UR EU gallery identified 33 (23%) multi-drug resistant ESBL-producing strains, while the double synergy test identified 35 strains without disc reconciliation and 38 strains after reconciliation. The most prevalent ESBL-E isolate was Escherichia coli, accounting for 77.1% of the isolates, followed by (20%) and (2.9%). Additionally, the study revealed the emergence of Imipenem resistance (5.7%), a critical last-resort antibiotic. However, all ESBL strains were sensitive to Fosfomycin (FSF/FOS), demonstrating its potential as an effective therapeutic option. Moreover, 37% of the ESBL producers exhibited co-resistance to over 20 different antibiotics. This study provides valuable insights into the prevalence and susceptibility patterns of ESBL-E associated with UTIs in Northern Cameroon. These insights emphasizes the importance of implementing appropriate treatment guidelines and antimicrobial stewardship measures to mitigate the spread and impact of MDR ESBL-producing strains on public health.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Sankar M., Rajalakshmi Engineering College, India; Viduthalai Rasheedkhan Regina, Nanyang Technological University, Singapore Edited by: Saravanan Periasamy, Rajalakshmi Engineering College, India
ISSN:	2296-2565 2296-2565
DOI:	10.3389/fpubh.2023.1187934