Search Results - "Enchev, Radoslav"

Protein neddylation: beyond cullin–RING ligases by Enchev, Radoslav I., Schulman, Brenda A., Peter, Matthias

“…Key Points NEDD8 (neural precursor cell expressed developmentally downregulated protein 8) and ubiquitin have the highest sequence and structural similarity…”
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Structural dynamics: review of time‐resolved cryo‐EM by Mäeots, Märt-Erik, Enchev, Radoslav I.

“…The structural determination of biological macromolecules has been transformative for understanding biochemical mechanisms and developing therapeutics…”
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A mechanism for the suppression of homologous recombination in G1 cells by Orthwein, Alexandre, Noordermeer, Sylvie M., Wilson, Marcus D., Landry, Sébastien, Enchev, Radoslav I., Sherker, Alana, Munro, Meagan, Pinder, Jordan, Salsman, Jayme, Dellaire, Graham, Xia, Bing, Peter, Matthias, Durocher, Daniel

“…A mechanism for the repression of homologous recombination in G1, the stage of the cell cycle preceding replication, is determined; the critical aspects are…”
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Modular microfluidics enables kinetic insight from time-resolved cryo-EM by Mäeots, Märt-Erik, Lee, Byungjin, Nans, Andrea, Jeong, Seung-Geun, Esfahani, Mohammad M. N., Ding, Shan, Smith, Daniel J., Lee, Chang-Soo, Lee, Sung Sik, Peter, Matthias, Enchev, Radoslav I.

“…Mechanistic understanding of biochemical reactions requires structural and kinetic characterization of the underlying chemical processes. However, no single…”
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Multilayered regulation of autophagy by the Atg1 kinase orchestrates spatial and temporal control of autophagosome formation by Schreiber, Anne, Collins, Ben C., Davis, Colin, Enchev, Radoslav I., Sedra, Angie, D’Antuono, Rocco, Aebersold, Ruedi, Peter, Matthias

“…Autophagy is a conserved intracellular degradation pathway exerting various cytoprotective and homeostatic functions by using de novo double-membrane vesicle…”
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Regulatory control of DNA end resection by Sae2 phosphorylation by Cannavo, Elda, Johnson, Dominic, Andres, Sara N., Kissling, Vera M., Reinert, Julia K., Garcia, Valerie, Erie, Dorothy A., Hess, Daniel, Thomä, Nicolas H., Enchev, Radoslav I., Peter, Matthias, Williams, R. Scott, Neale, Matt J., Cejka, Petr

“…DNA end resection plays a critical function in DNA double-strand break repair pathway choice. Resected DNA ends are refractory to end-joining mechanisms and…”
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Single-molecule analysis reveals cooperative stimulation of Rad51 filament nucleation and growth by mediator proteins by Belan, Ondrej, Barroso, Consuelo, Kaczmarczyk, Artur, Anand, Roopesh, Federico, Stefania, O’Reilly, Nicola, Newton, Matthew D., Maeots, Erik, Enchev, Radoslav I., Martinez-Perez, Enrique, Rueda, David S., Boulton, Simon J.

“…Homologous recombination (HR) is an essential DNA double-strand break (DSB) repair mechanism, which is frequently inactivated in cancer. During HR, RAD51 forms…”
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CSNAP, the smallest CSN subunit, modulates proteostasis through cullin-RING ubiquitin ligases by Füzesi-Levi, Maria G., Fainer, Irit, Ivanov Enchev, Radoslav, Ben-Nissan, Gili, Levin, Yishai, Kupervaser, Meital, Friedlander, Gilgi, Salame, Tomer Meir, Nevo, Reinat, Peter, Matthias, Sharon, Michal

“…The cullin-RING ubiquitin E3 ligase (CRL) family consists of ~250 complexes that catalyze ubiquitylation of proteins to achieve cellular regulation. All CRLs…”
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Polyubiquitin binding and cross-reactivity in the USP domain deubiquitinase USP21 by Ye, Yu, Akutsu, Masato, Reyes-Turcu, Francisca, Enchev, Radoslav I, Wilkinson, Keith D, Komander, David

“…Modification of proteins by ubiquitin (Ub) and Ub‐like (Ubl) modifiers regulates a variety of cellular functions. The ability of Ub to form chains of eight…”
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Structural Basis for a Reciprocal Regulation between SCF and CSN by Enchev, Radoslav I., Scott, Daniel C., da Fonseca, Paula C.A., Schreiber, Anne, Monda, Julie K., Schulman, Brenda A., Peter, Matthias, Morris, Edward P.

“…Skp1-Cul1-Fbox (SCF) E3 ligases are activated by ligation to the ubiquitin-like protein Nedd8, which is reversed by the deneddylating Cop9 signalosome (CSN)…”
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Structural and mechanistic insights into the CAND1-mediated SCF substrate receptor exchange by Shaaban, Mohammed, Clapperton, Julie A., Ding, Shan, Kunzelmann, Simone, Mäeots, Märt-Erik, Maslen, Sarah L., Skehel, J. Mark, Enchev, Radoslav I.

“…Modular SCF (SKP1-CUL1-Fbox) ubiquitin E3 ligases orchestrate multiple cellular pathways in eukaryotes. Their variable SKP1-Fbox substrate receptor (SR)…”
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Opposing effects of cancer-type-specific SPOP mutants on BET protein degradation and sensitivity to BET inhibitors by Janouskova, Hana, El Tekle, Geniver, Bellini, Elisa, Udeshi, Namrata D, Rinaldi, Anna, Ulbricht, Anna, Bernasocchi, Tiziano, Civenni, Gianluca, Losa, Marco, Svinkina, Tanya, Bielski, Craig M, Kryukov, Gregory V, Cascione, Luciano, Napoli, Sara, Enchev, Radoslav I, Mutch, David G, Carney, Michael E, Berchuck, Andrew, Winterhoff, Boris J N, Broaddus, Russell R, Schraml, Peter, Moch, Holger, Bertoni, Francesco, Catapano, Carlo V, Peter, Matthias, Carr, Steven A, Garraway, Levi A, Wild, Peter J, Theurillat, Jean-Philippe P

“…Different mutations found in endometrial and prostate tumors affecting the substrate-recognition domain of SPOP, a component of the E3 ubiquitin ligase…”
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Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection by Ferretti, Lorenza P., Himmels, Sarah-Felicitas, Trenner, Anika, Walker, Christina, von Aesch, Christine, Eggenschwiler, Aline, Murina, Olga, Enchev, Radoslav I., Peter, Matthias, Freire, Raimundo, Porro, Antonio, Sartori, Alessandro A.

“…Human CtIP is a decisive factor in DNA double-strand break repair pathway choice by enabling DNA-end resection, the first step that differentiates homologous…”
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Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase by Courtheoux, Thibault, Enchev, Radoslav I, Lampert, Fabienne, Gerez, Juan, Beck, Jochen, Picotti, Paola, Sumara, Izabela, Peter, Matthias

“…Directed cell movement involves spatial and temporal regulation of the cortical microtubule (Mt) and actin networks to allow focal adhesions (FAs) to assemble…”
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Structural basis for the subunit assembly of the anaphase-promoting complex by Schreiber, Anne, Stengel, Florian, Zhang, Ziguo, Enchev, Radoslav I., Kong, Eric H., Morris, Edward P., Robinson, Carol V., da Fonseca, Paula C. A., Barford, David

“…The anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions…”
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Structural and kinetic analysis of the COP9-Signalosome activation and the cullin-RING ubiquitin ligase deneddylation cycle by Mosadeghi, Ruzbeh, Reichermeier, Kurt M, Winkler, Martin, Schreiber, Anne, Reitsma, Justin M, Zhang, Yaru, Stengel, Florian, Cao, Junyue, Kim, Minsoo, Sweredoski, Michael J, Hess, Sonja, Leitner, Alexander, Aebersold, Ruedi, Peter, Matthias, Deshaies, Raymond J, Enchev, Radoslav I

“…The COP9-Signalosome (CSN) regulates cullin-RING ubiquitin ligase (CRL) activity and assembly by cleaving Nedd8 from cullins. Free CSN is autoinhibited, and it…”
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Structural Insights into the COP9 Signalosome and Its Common Architecture with the 26S Proteasome Lid and eIF3 by Enchev, Radoslav I., Schreiber, Anne, Beuron, Fabienne, Morris, Edward P.

“…The evolutionary conserved COP9 signalosome (CSN), a large multisubunit complex, plays a central role in regulating ubiquitination and cell signaling. Here we…”
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Cortical dynamics during cell motility are regulated by CRL3KLHL21 E3 ubiquitin ligase by Courtheoux, Thibault, Enchev, Radoslav I., Lampert, Fabienne, Gerez, Juan, Beck, Jochen, Picotti, Paola, Sumara, Izabela, Peter, Matthias

“…Directed cell movement involves spatial and temporal regulation of the cortical microtubule (Mt) and actin networks to allow focal adhesions (FAs) to assemble…”
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A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes by Gschweitl, Michaela, Ulbricht, Anna, Barnes, Christopher A, Enchev, Radoslav I, Stoffel-Studer, Ingrid, Meyer-Schaller, Nathalie, Huotari, Jatta, Yamauchi, Yohei, Greber, Urs F, Helenius, Ari, Peter, Matthias

“…Cullin-3 (CUL3)-based ubiquitin ligases regulate endosome maturation and trafficking of endocytic cargo to lysosomes in mammalian cells. Here, we report that…”
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Characterisation of the Cullin‐3 mutation that causes a severe form of familial hypertension and hyperkalaemia by Schumacher, Frances‐Rose, Siew, Keith, Zhang, Jinwei, Johnson, Clare, Wood, Nicola, Cleary, Sarah E, Al Maskari, Raya S, Ferryman, James T, Hardege, Iris, Yasmin, Figg, Nichola L, Enchev, Radoslav, Knebel, Axel, O'Shaughnessy, Kevin M, Kurz, Thimo

“…Deletion of exon 9 from Cullin‐3 (CUL3, residues 403–459: CUL3 Δ403–459 ) causes pseudohypoaldosteronism type IIE (PHA2E), a severe form of familial…”
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