Oseltamivir resistance mutation N294S in human influenza A(H5N1) virus in Egypt

Summary In December 2006, three human specimens were received that were suspected positive for influenza A(H5N1). The specimens were tested using real time PCR. And the presence of A(H5N1) virus was confirmed in 2 patients (16F and 26M), The NA sequence from A(H5N1) positive specimens collected befo...

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Published in:	Journal of infection and public health Vol. 2; no. 2; pp. 74 - 80
Main Authors:	Earhart, Kenneth C, Elsayed, Nasr M, Saad, Magdi D, Gubareva, Larisa V, Nayel, Ahmed, Deyde, Varough M, Abdelsattar, Ali, Abdelghani, Ahmad S, Boynton, Bruce R, Mansour, Moustafa M, Essmat, Hala M, Klimov, Alexander, Shuck-Lee, Deidra, Monteville, M.R, Tjaden, Jeffrey A
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 2009 Elsevier
Subjects:	A(H5N1) Adolescent Antiviral Agents - pharmacology Base Sequence Drug Resistance, Viral - drug effects Drug Resistance, Viral - genetics Egypt Female Humans Infectious Disease Influenza A virus Influenza A Virus, H5N1 Subtype - drug effects Influenza A Virus, H5N1 Subtype - genetics Influenza A Virus, H5N1 Subtype - isolation & purification Influenza, Human - drug therapy Influenza, Human - genetics Influenza, Human - virology Inhibitory Concentration 50 Internal Medicine Male Microbial Sensitivity Tests Mutation - genetics Neuraminidase - antagonists & inhibitors Neuraminidase - genetics Neuraminidase - metabolism Neuraminidase inhibitors Oseltamivir - pharmacology Oseltamivir resistance Phenotype Polymerase Chain Reaction RNA, Viral Young Adult Egypt Influenza A virus A(H5N1) Neuraminidase inhibitors Oseltamivir resistance Egypt
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Summary:	Summary In December 2006, three human specimens were received that were suspected positive for influenza A(H5N1). The specimens were tested using real time PCR. And the presence of A(H5N1) virus was confirmed in 2 patients (16F and 26M), The NA sequence from A(H5N1) positive specimens collected before and after antiviral therapy revealed a mutation (N294S) (N295S according to N1 numbering), previously associated with resistance to oseltamivir. When tested with NA inhibition assays, the two N294S viruses from Egypt exhibited from 57 to 138-fold reduction in susceptibility to oseltamivir, depending on the assay. To our knowledge, this is the first time oseltamivir resistance has been detected in A(H5N1) infecting a human prior to treatment.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1876-0341 1876-035X
DOI:	10.1016/j.jiph.2009.04.004