Nutrigenomic effect of conjugated linoleic acid on growth and meat quality indices of growing rabbit

Conjugated linoleic acid was detected in rabbit caecotrophs, due to the presence of microbial lipid activity in rabbit cecum. However, the effect of CLA as a functional food in growing rabbit is not well established. Therefore, this study was conducted to determine the effect of CLA on production, m...

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Published in:	PloS one Vol. 14; no. 10; p. e0222404
Main Authors:	Abdelatty, A M, Mohamed, Shereen A, Moustafa, Mahmoud M A, Al-Mokaddem, Asmaa K, Baker, M R, Elolimy, Ahmed A, Elmedany, Shawky A, Hussein, Shaymaa, Farid, Omar A A, Sakr, Osama G, Elhady, Mohamed A, Bionaz, Massimo
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 10-10-2019 Public Library of Science (PLoS)
Subjects:	Adipose tissue Adipose Tissue - drug effects Adipose Tissue - metabolism Animals Biology and Life Sciences Body Composition - drug effects Cecum Cell proliferation Diet Dietary Supplements Endocrinology Fatty acids Fatty Acids - metabolism Fatty Acids, Unsaturated - metabolism Fermentation Food Functional foods Functional foods & nutraceuticals Gene expression Genes Genetic engineering Health aspects Humans Kinases Linoleic acid Linoleic Acids, Conjugated - pharmacology Lipids Lipids - analysis Liver Liver - metabolism Meat Meat - analysis Meat quality Medicine and Health Sciences Metabolism Microorganisms Milk Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscles Musculoskeletal system Nutrigenomics Nutrition research Oils & fats Peroxisome proliferator-activated receptors Physiology Polyunsaturated fatty acids Proliferating cell nuclear antigen Proteins Rabbits Research and Analysis Methods Saturated fatty acids Skeletal muscle Studies Subcutaneous Fat - drug effects Subcutaneous Fat - metabolism Unsaturated fatty acids Veterinary medicine Oregon United States > US Egypt Giza Egypt
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Summary:	Conjugated linoleic acid was detected in rabbit caecotrophs, due to the presence of microbial lipid activity in rabbit cecum. However, the effect of CLA as a functional food in growing rabbit is not well established. Therefore, this study was conducted to determine the effect of CLA on production, meat quality, and its nutrigenomic effect on edible parts of rabbit carcass including skeletal muscle, liver, and adipose tissue. Therefore, seventy five weaned V-Line male rabbits, 30 days old, were randomly allocated into three dietary treatments receiving either basal control diet, diet supplemented with 0.5% (CLAL), or 1% CLA (CLAH). Total experimental period (63 d) was segmented into 7 days adaptation and 56 days experimental period. Dietary supplementation of CLA did not alter growth performance, however, the fat percentage of longissimus lumborum muscle was decreased, with an increase in protein and polyunsaturated fatty acids (PUFA) percentage. Saturated fatty acids (SFA) and mono unsaturated fatty acids (MUFA) were not increased in CLA treated groups. There was tissue specific sensing of CLA, since subcutaneous adipose tissue gene expression of PPARA was downregulated, however, CPT1A tended to be upregulated in liver of CLAL group only (P = 0.09). In skeletal muscle, FASN and PPARG were upregulated in CLAH group only (P ≤0.01). Marked cytoplasmic vacuolation was noticed in liver of CLAH group without altering hepatocyte structure. Adipocyte size was decreased in CLA fed groups, in a dose dependent manner (P <0.01). Cell proliferation determined by PCNA was lower (P <0.01) in adipose tissue of CLA groups. Our data indicate that dietary supplementation of CLA (c9,t11-CLA and t10,c12- CLA) at a dose of 0.5% in growing rabbit diet produce rabbit meat rich in PUFA and lower fat % without altering growth performance and hepatocyte structure.
Bibliography:	Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0222404