Search Results - "Ellefson, Jared W"

Synthetic evolutionary origin of a proofreading reverse transcriptase by Ellefson, Jared W., Gollihar, Jimmy, Shroff, Raghav, Shivram, Haridha, Iyer, Vishwanath R., Ellington, Andrew D.

“…Most reverse transcriptase (RT) enzymes belong to a single protein family of ancient evolutionary origin. These polymerases are inherently error prone, owing…”
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Directed evolution of a synthetic phylogeny of programmable Trp repressors by Ellefson, Jared W., Ledbetter, Michael P., Ellington, Andrew D.

“…As synthetic regulatory programs expand in sophistication, an ever increasing number of biological components with predictable phenotypes is required…”
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Directed evolution of genetic parts and circuits by compartmentalized partnered replication by Ellefson, Jared W, Meyer, Adam J, Hughes, Randall A, Cannon, Joe R, Brodbelt, Jennifer S, Ellington, Andrew D

“…A PCR-based directed evolution method facilitates selection of synthetic biology circuits and parts with desired functionality. Most existing directed…”
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Addicting diverse bacteria to a noncanonical amino acid by Tack, Drew S, Ellefson, Jared W, Thyer, Ross, Wang, Bo, Gollihar, Jimmy, Forster, Matthew T, Ellington, Andrew D

“…Expansion of the genetic code to noncanonical amino acids (NCAAs) has been limited by the lack of evolutionary pressure for organismal dependence on the NCAA…”
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Compartmentalized partnered replication for the directed evolution of genetic parts and circuits by Abil, Zhanar, Ellefson, Jared W, Gollihar, Jimmy D, Watkins, Ella, Ellington, Andrew D

“…This protocol describes the procedures for compartmentalized partnered replication (CPR), an emulsion-based directed evolution method for the generation of…”
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Cellular reagents for diagnostics and synthetic biology by Bhadra, Sanchita, Pothukuchy, Arti, Shroff, Raghav, Cole, Austin W, Byrom, Michelle, Ellefson, Jared W, Gollihar, Jimmy D, Ellington, Andrew D

“…We have found that the overproduction of enzymes in bacteria followed by their lyophilization leads to 'cellular reagents' that can be directly used to carry…”
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Identification of tumor-reactive B cells and systemic IgG in breast cancer based on clonal frequency in the sentinel lymph node by McDaniel, Jonathan R., Pero, Stephanie C., Voss, William N., Shukla, Girja S., Sun, Yujing, Schaetzle, Sebastian, Lee, Chang-Han, Horton, Andrew P., Harlow, Seth, Gollihar, Jimmy, Ellefson, Jared W., Krag, Christopher C., Tanno, Yuri, Sidiropoulos, Nikoletta, Georgiou, George, Ippolito, Gregory C., Krag, David N.

“…A better understanding of antitumor immune responses is the key to advancing the field of cancer immunotherapy. Endogenous immunity in cancer patients, such as…”
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Directed Evolution of a Panel of Orthogonal T7 RNA Polymerase Variants for in Vivo or in Vitro Synthetic Circuitry by Meyer, Adam J, Ellefson, Jared W, Ellington, Andrew D

“…T7 RNA polymerase is the foundation of synthetic biological circuitry both in vivo and in vitro due to its robust and specific control of transcription from…”
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Abiotic Self-Replication by Meyer, Adam J, Ellefson, Jared W, Ellington, Andrew D

“…The key to the origins of life is the replication of information. Linear polymers such as nucleic acids that both carry information and can be replicated are…”
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Bacteriophages use an expanded genetic code on evolutionary paths to higher fitness by Hammerling, Michael J, Ellefson, Jared W, Boutz, Daniel R, Marcotte, Edward M, Ellington, Andrew D, Barrick, Jeffrey E

“…Translational reprogramming, which enables site-specific incorporation of non-natural amino acids into proteins, offers practical tools for studying protein…”
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Recovery of Information Stored in Modified DNA with an Evolved Polymerase by Shroff, Raghav, Ellefson, Jared W, Wang, Siyuan S, Boulgakov, Alexander A, Hughes, Randall A, Ellington, Andrew D

“…DNA is increasingly being explored as an alternative medium for digital information storage, but the potential information loss from degradation and associated…”
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A facile technology for the high-throughput sequencing of the paired VH:VL and TCRβ:TCRα repertoires by Tanno, Hidetaka, McDaniel, Jonathan R, Stevens, Christopher A, Voss, William N, Li, Jie, Durrett, Russell, Lee, Jiwon, Gollihar, Jimmy, Tanno, Yuri, Delidakis, George, Pothukuchy, Arti, Ellefson, Jared W, Goronzy, Jörg J, Maynard, Jennifer A, Ellington, Andrew D, Ippolito, Gregory C, Georgiou, George

“…Natively paired sequencing (NPS) of B cell receptors [variable heavy (VH) and light (VL)] and T cell receptors (TCRb and TCRa) is essential for the…”
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Predicting Evolution of the Transcription Regulatory Network in a Bacteriophage by Garry, Daniel J, Meyer, Adam J, Ellefson, Jared W, Bull, James J, Ellington, Andrew D

“…Prediction of evolutionary trajectories has been an elusive goal, requiring a deep knowledge of underlying mechanisms that relate genotype to phenotype plus…”
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In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity by Martini, Laura, Meyer, Adam J, Ellefson, Jared W, Milligan, John N, Forlin, Michele, Ellington, Andrew D, Mansy, Sheref S

“…An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the…”
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Library generation by gene shuffling by Meyer, Adam J, Ellefson, Jared W, Ellington, Andrew D

“…This unit describes the process of gene shuffling, also known as sexual PCR. Gene shuffling is a facile method for the generation of sequence libraries…”
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An in vitro Autogene by Davidson, Eric A, Meyer, Adam J, Ellefson, Jared W, Levy, Matthew, Ellington, Andrew D

“…Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed…”
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Directed evolution of genetic parts and circuits by compartmentalized partnered replication. 9 mM Tris-HCl by Ellefson, Jared W, Meyer, Adam J, Hughes, Randall A, Cannon, Joe R, Brodbelt, Jennifer S, Ellington, Andrew D

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Directed evolution of genetic parts and circuits by compartmentalized partnered replication. 9 mM Tris-HCl (pH 8.3), 1.4 mM , 0.5 nM each CPR primer (JE.33 and JE.35) and 250 µM each dNTP by Ellefson, Jared W, Meyer, Adam J, Hughes, Randall A, Cannon, Joe R, Brodbelt, Jennifer S, Ellington, Andrew D

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High-throughput discovery of antitumor antibodies mined from sentinel lymph node B cells and circulating IgG of breast cancer patients by McDaniel, Jonathan R., Pero, Stephanie C., Voss, William N., Shukla, Girja S., Sun, Yujing, Schaetzle, Sebastian, Lee, Chang-Han, Horton, Andrew P., Harlow, Seth, Gollihar, Jimmy, Ellefson, Jared W., Krag, Christopher C., Tanno, Yuri, Sidiropoulos, Nikoletta, Georgiou, George, Ippolito, Gregory C., Krag, David N.

“…A better understanding of antitumor immune responses is key to advancing the field of cancer immunotherapy. Immune responses such as circulating autoantibodies…”
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