Search Results - "Elkins, Kelly M"
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Rapid Presumptive "Fingerprinting" of Body Fluids and Materials by ATR FT-IR Spectroscopy
Published in Journal of forensic sciences (01-11-2011)“…: Human body fluids and materials were evaluated using attenuated total reflectance Fourier transform infrared spectroscopy. Purified proteins, cosmetics, and…”
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The Differentiation of Menstrual from Venous Blood and Other Body Fluids on Various Substrates Using ATR FT‐IR Spectroscopy
Published in Journal of forensic sciences (01-01-2017)“…Crime scene investigators and laboratory analysts use chemical tests to detect and differentiate body fluids. Testing often requires a sample of the stain, and…”
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Development of Polymerase Chain Reaction-High-Resolution Melt Assay for Waterborne Pathogens Legionella pneumophila , Vibrio parahaemolyticus , and Camplobacter jejuni
Published in Microorganisms (Basel) (03-07-2024)“…is the waterborne pathogen primarily responsible for causing both Pontiac Fever and Legionnaire's Disease in humans. is transmitted via aerosolized water…”
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Simultaneous Identification of Four “Legal High” Plant Species in a Multiplex PCR High‐Resolution Melt Assay
Published in Journal of forensic sciences (01-05-2017)“…The international prevalence of “legal high” drugs necessitates the development of a method for their detection and identification. Herein, we describe the…”
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Comparison of proteases in DNA extraction via quantitative polymerase chain reaction
Published in Analytical biochemistry (01-06-2015)“…We compared four proteases in the QIAamp DNA Investigator Kit (Qiagen) to extract DNA for use in multiplex polymerase chain reaction (PCR) assays. The aim was…”
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High Resolution Melt Assays to Detect and Identify Vibrio parahaemolyticus , Bacillus cereus , Escherichia coli , and Clostridioides difficile Bacteria
Published in Microorganisms (Basel) (14-04-2020)“…Over one hundred bacterial species have been determined to comprise the human microbiota in a healthy individual. Bacteria including , , , and are found inside…”
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Pyrosequencing Primer Design for Forensic Biology Applications
Published in Methods in molecular biology (Clifton, N.J.) (2022)“…The polymerase chain reaction (PCR) is used to copy DNA in vitro for a variety of applications including amplifying a target DNA, mutating a base, adding tags,…”
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Additional predictions for forensic DNA phenotyping of externally visible characteristics using the ForenSeq and Imagen kits
Published in Journal of forensic sciences (01-03-2023)“…Multiplex DNA typing methods using massively parallel sequencing can be used to predict externally visible characteristics (EVCs) in forensic DNA phenotyping…”
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Detection and Identification of Kratom (Mitragyna speciosa) and Marijuana (Cannabis sativa) by a Real‐Time Polymerase Chain Reaction High‐Resolution Melt Duplex Assay
Published in Journal of forensic sciences (01-01-2020)“…Mitragyna speciosa (MS), a plant commonly known as kratom, is a widely used “legal high” opiate alternative for pain relief. DNA extracted from MS and 26…”
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Primer design for PCR reactions in forensic biology
Published in Methods in molecular biology (Clifton, N.J.) (2015)“…The polymerase chain reaction (PCR) is a popular method to copy DNA in vitro. Its invention revolutionized fields ranging from clinical medicine to…”
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Comparison of DNA yield and STR profiles from the diaphysis, mid‐diaphysis, and metaphysis regions of femur and tibia long bones
Published in Journal of forensic sciences (01-05-2021)“…DNA testing of human bones is performed for identification when there is no remaining soft tissue, which often means the samples are old or environmentally…”
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Development of a PCR High‐Resolution Melt Assay for Artemisia absinthium (Wormwood) and a Triplex Assay with Two Additional “Unregulated Legal High” Species Datura stramonium (Jimson Weed) and Merremia tuberosa (Hawaiian Woodrose)
Published in Journal of forensic sciences (01-11-2019)“…Artemisia absinthium (wormwood), a common ingredient in absinthe, contains the compound thujone, which is unregulated by the U.S. Drug Enforcement Agency…”
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Detection and Identification of Psilocybe cubensis DNA Using a Real‐Time Polymerase Chain Reaction High Resolution Melt Assay
Published in Journal of forensic sciences (01-09-2018)“…Psilocybe cubensis, or “magic mushroom,” is the most common species of fungus with psychedelic characteristics. Two primer sets were designed to target…”
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Evaluation of Two New Methods for DNA Extraction of “Legal High” Plant Species
Published in Journal of forensic sciences (01-09-2020)“…A quick, simple, and high‐yield nucleic acid isolation process is crucial for high‐quality DNA analysis. The ability of the MicroGEM PDQeX phytoGEM system and…”
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Colour quantitation for chemical spot tests for a controlled substances presumptive test database
Published in Drug testing and analysis (01-02-2017)“…Crime scene investigators (CSIs) often encounter unknown powders, capsules, tablets, and liquids at crime scenes, many of which are controlled substances…”
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Rapid and inexpensive species differentiation using a multiplex real-time polymerase chain reaction high-resolution melt assay
Published in Analytical biochemistry (01-05-2016)“…We demonstrate a method for developing real-time polymerase chain reaction (PCR) high-resolution melt (HRM) assays to identify multiple species present in a…”
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Designing Polymerase Chain Reaction (PCR) Primer Multiplexes in the Forensic Laboratory
Published in Journal of chemical education (01-10-2011)“…The polymerase chain reaction (PCR) is a common experiment in upper-level undergraduate biochemistry, molecular biology, and forensic laboratory courses as…”
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Curriculum and course materials for a forensic DNA biology course
Published in Biochemistry and molecular biology education (01-01-2014)“…The Forensic Science Education Programs Accreditation Commission (FEPAC) requires accredited programs offer a “coherent curriculum” to ensure each student…”
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Assessing DNA recovery from chewing gum
Published in Medicine, science, and the law (01-01-2017)“…The purpose of this study was to evaluate which DNA extraction method yields the highest quantity of DNA from chewing gum. In this study, several popular…”
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An Undergraduate Laboratory Experiment for Upper-Level Forensic Science, Biochemistry, or Molecular Biology Courses: Human DNA Amplification Using STR Single Locus Primers by Real-Time PCR with SYBR Green Detection
Published in Journal of chemical education (08-05-2012)“…In this laboratory experiment, real-time polymerase chain reaction (real-time PCR) was conducted using published human TPOX single-locus DNA primers for…”
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